Gene expression profiling of the Cephalothorax and Eyestalk in Penaeus Monodon during Ovarian Maturation

Brady, Philip, Elizur, Abigail, Williams, Richard, Cummins, Scott F. and Knibb, Wayne (2012) Gene expression profiling of the Cephalothorax and Eyestalk in Penaeus Monodon during Ovarian Maturation. International Journal of Biological Sciences, 8 3: 328-343. doi:10.7150/ijbs.3517

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Author Brady, Philip
Elizur, Abigail
Williams, Richard
Cummins, Scott F.
Knibb, Wayne
Title Gene expression profiling of the Cephalothorax and Eyestalk in Penaeus Monodon during Ovarian Maturation
Journal name International Journal of Biological Sciences   Check publisher's open access policy
ISSN 1449-2288
Publication date 2012
Sub-type Article (original research)
DOI 10.7150/ijbs.3517
Open Access Status DOI
Volume 8
Issue 3
Start page 328
End page 343
Total pages 16
Place of publication Lake Haven, NSW, Australia
Publisher Ivyspring International Publisher
Collection year 2013
Language eng
Abstract In crustaceans, a range of physiological processes involved in ovarian maturation occurs in organs of the cephalothorax including the hepatopancrease, mandibular and Y-organ. Additionally, reproduction is regulated by neuropeptide hormones and other proteins released from secretory sites within the eyestalk. Reproductive dysfunction in captive-reared prawns, Penaeus monodon, is believed to be due to deficiencies in these factors. In this study, we investigated the expression of gene transcripts in the cephalothorax and eyestalk from wild-caught and captive-reared animals throughout ovarian maturation using custom oligonucleotide microarray screening. We have isolated numerous transcripts that appear to be differentially expressed throughout ovarian maturation and between wild-caught and captive-reared animals. In the cephalothorax, differentially expressed genes included the 1,3-β-D-glucan-binding high-density lipoprotein, 2/3-oxoacyl-CoA thiolase and vitellogenin. In the eyestalk, these include gene transcripts that encode a protein that modulates G-protein coupled receptor activity and another that encodes an architectural transcription factor. Each may regulate the expression of reproductive neuropeptides, such as the crustacean hyperglycaemic hormone and molt-inhibiting hormone. We could not identify differentially expressed transcripts encoding known reproductive neuropeptides in the eyestalk of either wild-caught or captive-reared prawns at any ovarian maturation stage, however, this result may be attributed to low relative expression levels of these transcripts. In summary, this study provides a foundation for the study of target genes involved in regulating penaeid reproduction.
Keyword Cephalothorax
Gene expression
Ovarian maturation
Penaeus monodon
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Agriculture and Food Sciences
Official 2013 Collection
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Citation counts: TR Web of Science Citation Count  Cited 8 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 9 times in Scopus Article | Citations
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Created: Mon, 02 Apr 2012, 11:27:19 EST by System User on behalf of School of Agriculture and Food Sciences