In vivo imaging and biodistribution of multimodal polymeric nanoparticles delivered to the optic nerve

Harrison, James, Bartlett, Carole A., Cowin, Gary, Nicholls, Philip K., Evans, Cameron W., Clemons, Tristan D., Zdyrko, Bogdan, Luzinov, Igor A., Harvey, Alan R., Iyer, K. Swaminathan, Dunlop, Sarah A. and Fitzgerald, Melinda (2012) In vivo imaging and biodistribution of multimodal polymeric nanoparticles delivered to the optic nerve. Small, 8 10: 1579-1589. doi:10.1002/smll.201102648

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Author Harrison, James
Bartlett, Carole A.
Cowin, Gary
Nicholls, Philip K.
Evans, Cameron W.
Clemons, Tristan D.
Zdyrko, Bogdan
Luzinov, Igor A.
Harvey, Alan R.
Iyer, K. Swaminathan
Dunlop, Sarah A.
Fitzgerald, Melinda
Title In vivo imaging and biodistribution of multimodal polymeric nanoparticles delivered to the optic nerve
Journal name Small   Check publisher's open access policy
ISSN 1613-6810
1613-6829
Publication date 2012-05-21
Sub-type Article (original research)
DOI 10.1002/smll.201102648
Volume 8
Issue 10
Start page 1579
End page 1589
Total pages 11
Place of publication Weinheim, Germany
Publisher Wiley
Collection year 2013
Language eng
Formatted abstract
The use of nanoparticles for targeted delivery of therapeutic agents to sites of injury or disease in the central nervous system (CNS) holds great promise. However, the biodistribution of nanoparticles following in vivo administration is often unknown, and concerns have been raised regarding potential toxicity. Using poly(glycidyl methacrylate) (PGMA) nanoparticles coated with polyethylenimine (PEI) and containing superparamagnetic iron oxide nanoparticles as a magnetic resonance imaging (MRI) contrast agent and rhodamine B as a fluorophore, whole animal MRI and fluorescence analyses are used to demonstrate that these nanoparticles (NP) remain close to the site of injection into a partial injury of the optic nerve, a CNS white matter tract. In addition, some of these NP enter axons and are transported to parent neuronal somata. NP also remain in the eye following intravitreal injection, a non-injury model. Considerable infiltration of activated microglia/macrophages occurs in both models. Using magnetic concentration and fluorescence visualization of tissue homogenates, no dissemination of the NP into peripheral tissues is observed. Histopathological analysis reveals no toxicity in organs other than at the injection sites. Multifunctional nanoparticles may be a useful mechanism to deliver therapeutic agents to the injury site and somata of injured CNS neurons and thus may be of therapeutic value following brain or spinal cord trauma.
Keyword Nanoparticles
In vivo imaging
Magnetism
Fluorescence
Biomedical applications
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
Centre for Advanced Imaging Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 21 times in Thomson Reuters Web of Science Article | Citations
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Created: Mon, 26 Mar 2012, 11:25:04 EST by Sandrine Ducrot on behalf of Centre for Advanced Imaging