Isolation and characterisation of novel microsatellite and mitochondrial DNA markers for the Eastern Water Dragon (Physignathus lesueurii)

Frere, C. H., Prentis, P. J., Ezaz, T. and Georges, A. (2012) Isolation and characterisation of novel microsatellite and mitochondrial DNA markers for the Eastern Water Dragon (Physignathus lesueurii). Conservation Genetics Resources, 4 1: 113-116. doi:10.1007/s12686-011-9487-3


Author Frere, C. H.
Prentis, P. J.
Ezaz, T.
Georges, A.
Title Isolation and characterisation of novel microsatellite and mitochondrial DNA markers for the Eastern Water Dragon (Physignathus lesueurii)
Formatted title
Isolation and characterisation of novel microsatellite and mitochondrial DNA markers for the Eastern Water Dragon (Physignathus lesueurii)
Journal name Conservation Genetics Resources   Check publisher's open access policy
ISSN 1877-7252
1877-7260
Publication date 2012-03
Year available 2011
Sub-type Article (original research)
DOI 10.1007/s12686-011-9487-3
Volume 4
Issue 1
Start page 113
End page 116
Total pages 4
Place of publication Dordrecht, Netherlands
Publisher Springer Netherlands
Collection year 2012
Language eng
Formatted abstract
Habitat fragmentation as a result of urbanisation is a growing problem for native lizard species. The Eastern Water Dragon (Physignathus lesueurii) is a social arboreal agamid lizard, native to Australia. This species represents an ideal model species to investigate the effect of urbanisation because of their prominent abundance in the urban landscape. Here we describe the isolation and characterisation of a novel set of 74 di-, tri-, and tetra-microsatellites from which 18 were selected and optimised into two multiplexes. The 18 microsatellites generated a total 148 alleles across the two populations. The number of alleles per locus varied from 2 to 18 alleles and measures of Ho and He varied from 0.395 to 0.877 and from 0.441 to 0.880, respectively. We also present primers for four novel mitochondrial DNA (mtDNA) markers. The combined length of the four mtDNA marker pairs was 2,528 bp which included 15 nucleotides changes. In comparison to threatened species, which are generally characterised by small population sizes, the Eastern Water Dragon represents an ideal model species to investigate the effect of urbanisation on their behavioural ecology and connectivity patterns among populations.
Keyword Paternity
Agamid lizards
Agamidae
Gene flow
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online: 27 July 2011. Published under Technical Note.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
School of Biological Sciences Publications
 
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Created: Fri, 23 Mar 2012, 17:42:44 EST by Gail Walter on behalf of School of Biological Sciences