Engineering a mammalian super producer

Dietmair, Stefanie, Nielsen, Lars K. and Timmins, Nicholas E. (2011) Engineering a mammalian super producer. Journal of Chemical Technology and Biotechnology, 86 7: 905-914. doi:10.1002/jctb.2576


Author Dietmair, Stefanie
Nielsen, Lars K.
Timmins, Nicholas E.
Title Engineering a mammalian super producer
Journal name Journal of Chemical Technology and Biotechnology   Check publisher's open access policy
ISSN 0268-2575
1097-4660
Publication date 2011-07
Sub-type Critical review of research, literature review, critical commentary
DOI 10.1002/jctb.2576
Volume 86
Issue 7
Start page 905
End page 914
Total pages 10
Place of publication West Sussex, United Kingdom
Publisher John Wiley & Sons
Collection year 2012
Language eng
Abstract Mammalian cells are the preferred host for the manufacture of a wide range of biopharmaceuticals, but production costs are high owing to low productivity. A range of rational engineering strategies have been pursued in order to increase volumetric product titres from mammalian cells, such as delaying apoptosis, manipulation of the cell cycle, and improving metabolism and protein processing. Unfortunately, outcomes from these strategies have been mixed, with few instances where significant improvements in product yield have been achieved. This article reviews and contrasts many of the engineering strategies attempted to date, highlighting the variability and context specificity in outcome. The paper argues that this is a reflection of the complexity of mammalian cells, and that a deeper understanding of the biology underpinning protein production for biotechnological purposes is required.
Keyword Host cell engineering
Mammalian cell
Review
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Article first published online: 15 February 2011.

Document type: Journal Article
Sub-type: Critical review of research, literature review, critical commentary
Collections: Official 2012 Collection
Australian Institute for Bioengineering and Nanotechnology Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 14 times in Thomson Reuters Web of Science Article | Citations
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Created: Wed, 21 Mar 2012, 10:42:41 EST by Ms Lynette Adams on behalf of Aust Institute for Bioengineering & Nanotechnology