A transcriptomic analysis of the adult stage of the bovine lungworm, Dictyocaulus viviparus

Ranganathan, Shoba, Nagaraj, Shivashankar H., Hu, Min, Strube, Christina, Schnieder, Thomas and Gasser, Robin B. (2007) A transcriptomic analysis of the adult stage of the bovine lungworm, Dictyocaulus viviparus. BMC Genomics, 8 5: 311-1-311-13. doi:10.1186/1471-2164-8-311

Author Ranganathan, Shoba
Nagaraj, Shivashankar H.
Hu, Min
Strube, Christina
Schnieder, Thomas
Gasser, Robin B.
Title A transcriptomic analysis of the adult stage of the bovine lungworm, Dictyocaulus viviparus
Formatted title
A transcriptomic analysis of the adult stage of the bovine lungworm, Dictyocaulus viviparus
Journal name BMC Genomics   Check publisher's open access policy
ISSN 1471-2164
Publication date 2007-09
Sub-type Article (original research)
DOI 10.1186/1471-2164-8-311
Open Access Status DOI
Volume 8
Issue 5
Start page 311-1
End page 311-13
Total pages 13
Place of publication London, United Kingdom
Publisher BioMed Central
Language eng
Formatted abstract
Lungworms of the genus Dictyocaulus (family Dictyocaulidae) are parasitic nematodes of major economic importance. They cause pathological effects and clinical disease in various ruminant hosts, particularly in young animals. Dictyocaulus viviparus, called the bovine lungworm, is a major pathogen of cattle, with severe infections being fatal. In this study, we provide first insights into the transcriptome of the adult stage of D. viviparus through the analysis of expressed sequence tags (ESTs).

Using our EST analysis pipeline, we estimate that the present dataset of 4436 ESTs is derived from 2258 genes based on cluster and comparative genomic analyses of the ESTs. Of the 2258 representative ESTs, 1159 (51.3%) had homologues in the free-living nematode C. elegans, 1174 (51.9%) in parasitic nematodes, 827 (36.6%) in organisms other than nematodes, and 863 (38%) had no significant match to any sequence in the current databases. Of the C. elegans homologues, 569 had observed 'non-wildtype' RNAi phenotypes, including embryonic lethality, maternal sterility, sterility in progeny, larval arrest and slow growth. We could functionally classify 776 (35%) sequences using the Gene Ontologies (GO) and established pathway associations to 696 (31%) sequences in Kyoto Encyclopedia of Genes and Genomes (KEGG). In addition, we predicted 85 secreted proteins which could represent potential candidates for developing novel anthelmintics or vaccines.


The bioinformatic analyses of ESTs data for D. viviparus has elucidated sets of relatively conserved and potentially novel genes. The genes discovered in this study should assist research toward a better understanding of the basic molecular biology of D. viviparus, which could lead, in the longer term, to novel intervention strategies. The characterization of the D. viviparus transcriptome also provides a foundation for whole genome sequence analysis and future comparative transcriptomic analyses.
Keyword Suppressive-subtractive hybridization
Nematode meloidogyne-incognita
Expressed sequence tags
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes Article number 311

Document type: Journal Article
Sub-type: Article (original research)
Collection: Institute for Molecular Bioscience - Publications
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Citation counts: TR Web of Science Citation Count  Cited 16 times in Thomson Reuters Web of Science Article | Citations
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Created: Wed, 21 Mar 2012, 09:57:17 EST by Susan Allen on behalf of Institute for Molecular Bioscience