Proteomics analysis of the excretory/secretory component of the blood-feeding stage of the hookworm, Ancylostoma caninum

Mulvenna, Jason, Hamilton, Brett, Nagaraj, Shivashankar H., Smyth, Danielle, Loukas, Alex and Gorman, Jeffrey J. (2009) Proteomics analysis of the excretory/secretory component of the blood-feeding stage of the hookworm, Ancylostoma caninum. Molecular and Cellular Proteomics, 8 1: 109-121. doi:10.1074/mcp.M800206-MCP200

Author Mulvenna, Jason
Hamilton, Brett
Nagaraj, Shivashankar H.
Smyth, Danielle
Loukas, Alex
Gorman, Jeffrey J.
Title Proteomics analysis of the excretory/secretory component of the blood-feeding stage of the hookworm, Ancylostoma caninum
Journal name Molecular and Cellular Proteomics   Check publisher's open access policy
ISSN 1535-9476
Publication date 2009-01
Sub-type Article (original research)
DOI 10.1074/mcp.M800206-MCP200
Volume 8
Issue 1
Start page 109
End page 121
Total pages 13
Place of publication Bethesda, MD, United States
Publisher merican Society for Biochemistry and Molecular Biology
Language eng
Abstract Hookworms are blood-feeding intestinal parasites of mammalian hosts and are one of the major human ailments affecting ∼600 million people worldwide. These parasites form an intimate association with the host and are able to avoid vigorous immune responses in many ways including skewing of the response phenotype to promote parasite survival and longevity. The primary interface between the parasite and the host is the excretory/secretory component, a complex mixture of proteins, carbohydrates, and lipids secreted from the surface or oral openings of the parasite. The composition of this complex mixture is for the most part unknown but is likely to contain proteins important for the parasitic lifestyle and hence suitable as drug or vaccine targets. Using a strategy combining the traditional technology of one-dimensional SDS-PAGE and the newer fractionation technology of OFFGEL electrophoresis we identified 105 proteins from the excretory/secretory products of the blood-feeding stage of the dog hookworm, Ancylostoma caninum. Highly represented among the identified proteins were lectins, including three C-type lectins and three β-galactoside-specific S-type galectins, as well as a number of proteases belonging to the three major classes found in nematodes, aspartic, cysteine, and metalloproteases. Interestingly 28% of the identified proteins were homologous to activation-associated secreted proteins, a family of cysteine-rich secreted proteins belonging to the sterol carrier protein/ Tpx-1/Ag5/PR-1/Sc-7 (TAPS) superfamily. Thirty-four of these proteins were identified suggesting an important role in host-parasite interactions. Other protein families identified included hyaluronidases, lysozyme-like proteins, and transthyretin-like proteins. This work identified a suite of proteins important for the parasitic lifestyle and provides new insight into the biology of hookworm infection.
Keyword Excretory-secretory products
Daspartic proteases
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Institute for Molecular Bioscience - Publications
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Created: Wed, 21 Mar 2012, 09:54:48 EST by Susan Allen on behalf of Institute for Molecular Bioscience