Genome-wide patterns of promoter sharing and co-expression in bovine skeletal muscle

Gu, Quan, Nagaraj, Shivashankar H., Hudson, Nicholas J., Dalrymple, Brian P. and Reverter, Antonio (2011) Genome-wide patterns of promoter sharing and co-expression in bovine skeletal muscle. BMC Genomics, 12 23.1-23.13. doi:10.1186/1471-2164-12-23

Author Gu, Quan
Nagaraj, Shivashankar H.
Hudson, Nicholas J.
Dalrymple, Brian P.
Reverter, Antonio
Title Genome-wide patterns of promoter sharing and co-expression in bovine skeletal muscle
Journal name BMC Genomics   Check publisher's open access policy
ISSN 1471-2164
Publication date 2011-01
Sub-type Article (original research)
DOI 10.1186/1471-2164-12-23
Open Access Status DOI
Volume 12
Start page 23.1
End page 23.13
Total pages 13
Place of publication London, England, U.K.
Publisher BioMed Central
Collection year 2012
Language eng
Formatted abstract
Gene regulation by transcription factors (TF) is species, tissue and time specific. To better understand how the genetic code controls gene expression in bovine muscle we associated gene expression data from developing Longissimus thoracis et lumborum skeletal muscle with bovine promoter sequence information.

We created a highly conserved genome-wide promoter landscape comprising 87,408 interactions relating 333 TFs with their 9,242 predicted target genes (TGs). We discovered that the complete set of predicted TGs share
an average of 2.75 predicted TF binding sites (TFBSs) and that the average co-expression between a TF and its predicted TGs is higher than the average co-expression between the same TF and all genes. Conversely, pairs of
TFs sharing predicted TGs showed a co-expression correlation higher that pairs of TFs not sharing TGs. Finally, we exploited the co-occurrence of predicted TFBS in the context of muscle-derived functionally-coherent modules including cell cycle, mitochondria, immune system, fat metabolism, muscle/glycolysis, and ribosome. Our findings enabled us to reverse engineer a regulatory network of core processes, and correctly identified the involvement of E2F1, GATA2 and NFKB1 in the regulation of cell cycle, fat, and muscle/glycolysis, respectively.

The pivotal implication of our research is two-fold: (1) there exists a robust genome-wide expression signal between TFs and their predicted TGs in cattle muscle consistent with the extent of promoter sharing; and (2) this signal can be exploited to recover the cellular mechanisms underpinning transcription regulation of muscle structure and development in bovine. Our study represents the first genome-wide report linking tissue specific coexpression to co-regulation in a non-model vertebrate.
Keyword Factor-binding Sites
Transcription Factors
Gene coexpression networks
Expression profiles
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
Queensland Alliance for Agriculture and Food Innovation
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Citation counts: TR Web of Science Citation Count  Cited 7 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 8 times in Scopus Article | Citations
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Created: Wed, 21 Mar 2012, 09:27:06 EST by Susan Allen on behalf of Qld Alliance for Agriculture and Food Innovation