Back to BAC: The use of infectious clone technologies for viral mutagenesis

Hall, Robyn N., Meers, Joanne, Fowler, Elizabeth and Mahony, Timothy (2012) Back to BAC: The use of infectious clone technologies for viral mutagenesis. Viruses, 4 2: 211-235.

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Author Hall, Robyn N.
Meers, Joanne
Fowler, Elizabeth
Mahony, Timothy
Title Back to BAC: The use of infectious clone technologies for viral mutagenesis
Journal name Viruses   Check publisher's open access policy
ISSN 1999-4915
Publication date 2012-02
Sub-type Critical review of research, literature review, critical commentary
DOI 10.3390/v4020211
Volume 4
Issue 2
Start page 211
End page 235
Total pages 25
Place of publication Basel, Switzerland
Publisher M D P I AG
Collection year 2013
Language eng
Abstract Bacterial artificial chromosome (BAC) vectors were first developed to facilitate the propagation and manipulation of large DNA fragments in molecular biology studies for uses such as genome sequencing projects and genetic disease models. To facilitate these studies, methodologies have been developed to introduce specific mutations that can be directly applied to the mutagenesis of infectious clones (icBAC) using BAC technologies. This has resulted in rapid identification of gene function and expression at unprecedented rates. Here we review the major developments in BAC mutagenesis in vitro. This review summarises the technologies used to construct and introduce mutations into herpesvirus icBAC. It also explores developing technologies likely to provide the next leap in understanding these important viruses.
Keyword Chromosomes
Artificial
Bacterial
Recombination
Genetic
Mutagenesis
Cloning
Molecular methods
Transposition
DNA viruses
Infectious clone
Q-Index Code CX
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published: 3 February 2012.

Document type: Journal Article
Sub-type: Critical review of research, literature review, critical commentary
Collections: Non HERDC
Queensland Alliance for Agriculture and Food Innovation
School of Medicine Publications
School of Veterinary Science Publications
 
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