Morphological and physiological response of soybean treated with the microsymbiont Bradyrhizobium japonicum pre-incubated with genistein

Dolatabadian, A., Sanavy, S.A.M.M., Ghanati, F. and Gresshoff, P. M. (2012) Morphological and physiological response of soybean treated with the microsymbiont Bradyrhizobium japonicum pre-incubated with genistein. South African Journal of Botany, 79 9-18. doi:10.1016/j.sajb.2011.11.001


Author Dolatabadian, A.
Sanavy, S.A.M.M.
Ghanati, F.
Gresshoff, P. M.
Title Morphological and physiological response of soybean treated with the microsymbiont Bradyrhizobium japonicum pre-incubated with genistein
Journal name South African Journal of Botany   Check publisher's open access policy
ISSN 0254-6299
1727-9321
Publication date 2012-03
Year available 2011
Sub-type Article (original research)
DOI 10.1016/j.sajb.2011.11.001
Volume 79
Start page 9
End page 18
Total pages 10
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Collection year 2012
Language eng
Abstract Genistein, a major root-secreted isoflavone of soybean (. Glycine max (L.) Merr), is critical for the legume-. Bradyrhizobium symbiosis as it induces several bacterial . nod-gene systems. An experiment with soybean grown under salt stress was conducted to evaluate the effect of exogenous genistein addition to the . Bradyrhizobium culture medium on subsequent nodulation, nitrogen fixation and selected plant physiological attributes. Five day-old plants (in pots) were inoculated with a liquid . B. japonicum broth culture and irrigated with B&D solution containing either 0, 25, 50 and 100. mM NaCl. Four weeks after inoculation, maximum photochemical efficiency of PSII (Fv/Fm), photosynthetic rate, stomatal conductance, and transpiration rate were measured. Number of nodules per plant and apparent nitrogen fixation (as acetylene reduction activity) were determined. Salt stress decreased nodule number/plant and nitrogenase activity/plant and induced large changes of both photosynthetic parameters and antioxidant enzyme activity, compared to the control, genistein reversed the effect in each level of salinity tested. Moreover, pre-treatment of the microsymbiont with genistein enhanced maximum photochemical efficiency, photosynthetic rate, stomatal conductance and transpiration rate, while the enzymatic activities of catalase, superoxide dismutase and peroxidase in leaves and roots were not affected. It can be concluded that preincubation of the . B. japonicum inoculant with genistein probably contributed towards growth in soybean via enhancement of nodulation and nitrogen fixation under both normal and salt stress conditions.
Keyword Antioxidant enzymes
Development
Isoflavone
Photosynthetic parameters
Salinity
Symbiosis
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online 16 December 2011.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Centre for Integrative Legume Research Publications
Official 2012 Collection
 
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Created: Fri, 16 Mar 2012, 11:07:02 EST by Susan O'Brien on behalf of Centre for Integrative Legume Research