Effects of a Lactobacillus reuteri BR11 mutant deficient in the cystine-transport system in a rat model of inflammatory bowel disease

Atkins, Haydn L., Geier, Mark S., Prisciandaro, Luca D., Pattanaik, Ashok K., Forder, Rebecca E. A., Turner, Mark S. and Howarth, Gordon S. (2012) Effects of a Lactobacillus reuteri BR11 mutant deficient in the cystine-transport system in a rat model of inflammatory bowel disease. Digestive Diseases and Sciences, 57 3: 713-719. doi:10.1007/s10620-011-1943-0


Author Atkins, Haydn L.
Geier, Mark S.
Prisciandaro, Luca D.
Pattanaik, Ashok K.
Forder, Rebecca E. A.
Turner, Mark S.
Howarth, Gordon S.
Title Effects of a Lactobacillus reuteri BR11 mutant deficient in the cystine-transport system in a rat model of inflammatory bowel disease
Formatted title
Effects of a Lactobacillus reuteri BR11 mutant deficient in the cystine-transport system in a rat model of inflammatory bowel disease
Journal name Digestive Diseases and Sciences   Check publisher's open access policy
ISSN 0163-2116
1573-2568
Publication date 2012-03
Year available 2011
Sub-type Article (original research)
DOI 10.1007/s10620-011-1943-0
Volume 57
Issue 3
Start page 713
End page 719
Total pages 7
Place of publication New York, United States
Publisher Springer
Collection year 2012
Language eng
Formatted abstract
Background: Inflammatory bowel disease (IBD) is a chronic inflammatory disorder of the gastrointestinal tract associated with altered composition of the gut microbiota. Lactobacillus reuteri BR11 (BR11) has recently been reported to reduce the severity of experimental IBD because of its probiotic properties possibly attributed to a mechanism of thiol production via its unique cysteine/cystine-transport system.
Aim: We compared BR11 and a BR11 mutant deficient in the cystine-uptake system (PNG201), for their capacity to reduce the severity of experimental colitis.
Methods: Male Sprague–Dawley rats (n = 8 per group) were gavaged (1 ml/day) with skim milk, BR11 or PNG201 (1 × 109 CFU/ml) for 12 days. Rats consumed either water or 2% dextran sulfate sodium in drinking water from days 6 to 12 to induce colitis. Metabolism data, disease activity index, intestinal mucin profile, and histological analyses were assessed and compared by ANOVA.
Results: Assessed histologically, DSS administration resulted in significant colonic deterioration, including loss of crypt area and increased damage severity. BR11 administration only partially alleviated the DSS effects, with a minor improvement in crypt area (P < 0.05). Administration of the PNG201 mutant strain to colitic animals failed to achieve significance (P > 0.05) against the DSS control for any of the end-points. However, the mutant strain induced significantly greater (P < 0.05) histological severity compared with BR11-treated colitic animals, indicative of possible exacerbation of colitis.
Conclusions: The cystine-uptake system only minimally affects the biological effects of BR11, as evidenced by histological and macroscopic colitic changes.
Keyword Colon
Dextran sulfate sodium
Inflammatory bowel disease
Lactobacillus reuteri
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online: 26 October 2011

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
School of Agriculture and Food Sciences
 
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Created: Thu, 15 Mar 2012, 15:48:29 EST by Dr Mark Turner on behalf of Qld Alliance for Agriculture and Food Innovation