Genome structure affects the rate of autosyndesis and allosyndesis in AABC, BBAC and CCAB Brassica interspecific hybrids

Mason, Annaliese S., Huteau, Virginie, Eber, Frédérique, Coriton, Olivier, Yan, Guijun, Nelson, Matthew N., Cowling, Wallace A. and Chèvre, Anne-Marie (2010) Genome structure affects the rate of autosyndesis and allosyndesis in AABC, BBAC and CCAB Brassica interspecific hybrids. Chromosome Research, 18 6: 655-666. doi:10.1007/s10577-010-9140-0


Author Mason, Annaliese S.
Huteau, Virginie
Eber, Frédérique
Coriton, Olivier
Yan, Guijun
Nelson, Matthew N.
Cowling, Wallace A.
Chèvre, Anne-Marie
Title Genome structure affects the rate of autosyndesis and allosyndesis in AABC, BBAC and CCAB Brassica interspecific hybrids
Formatted title
Genome structure affects the rate of autosyndesis and allosyndesis in AABC, BBAC and CCAB Brassica interspecific hybrids
Journal name Chromosome Research   Check publisher's open access policy
ISSN 0967-3849
1573-6849
Publication date 2010-09
Sub-type Article (original research)
DOI 10.1007/s10577-010-9140-0
Volume 18
Issue 6
Start page 655
End page 666
Total pages 12
Place of publication Dordrecht, Netherlands
Publisher Springer
Language eng
Formatted abstract
Gene introgression into allopolyploid crop species from diploid or polyploid ancestors can be accomplished through homologous or homoeologous chromosome pairing during meiosis. We produced trigenomic Brassica interspecific hybrids (genome complements AABC, BBAC and CCAB) from the amphidiploid species Brassica napus (AACC), Brassica juncea (AABB) and Brassica carinata (BBCC) in order to test whether the structure of each genome affects frequencies of homologous and homoeologous (both allosyndetic and autosyndetic) pairing during meiosis. AABC hybrids produced from three genotypes of B. napus were included to assess the genetic control of homoeologous pairing. Multi-colour fluorescent in situ hybridisation was used to quantify homologous pairing (e.g. A-genome bivalents in AABC), allosyndetic associations (e.g. B–C in AABC) and autosyndetic associations (e.g. B–B in AABC) at meiosis. A high percentage of homologous chromosomes formed pairs (97.5–99.3%), although many pairs were also involved in autosyndetic and allosyndetic associations. Allosyndesis was observed most frequently as A–C genome associations (mean 4.0 per cell) and less frequently as A–B genome associations (0.8 per cell) and B–C genome associations (0.3 per cell). Autosyndesis occurred most frequently in the haploid A genome (0.75 A–A per cell) and least frequently in the haploid B genome (0.13 B–B per cell). The frequency of C–C autosyndesis was greater in BBAC hybrids (0.75 per cell) than in any other hybrid. The frequency of A–B, A–C and B–C allosyndesis was affected by the genomic structure of the trigenomic hybrids. Frequency of allosyndesis was also influenced by the genotype of the B. napus paternal parent for the three AABC (B. juncea × B. napus) hybrid types. Homoeologous pairing between the Brassica A, B and C genomes in interspecific hybrids may be influenced by complex interactions between genome structure and allelic composition.
Keyword Allosyndesis
Autosyndesis
Meiosis
Brassica
Interspecific hybrid
Fluorescent in situ hybridisation
Oilseed rape
Leptosphaeria-maculans
Genetic diversity
Napus haploids
Homeologous recombination
Major role
Resistance
Identification
Introgression
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Agriculture and Food Sciences
 
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Citation counts: TR Web of Science Citation Count  Cited 27 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 30 times in Scopus Article | Citations
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Created: Thu, 15 Mar 2012, 11:19:53 EST by Annaliese Mason on behalf of School of Agriculture and Food Sciences