Microspore culture preferentially selects unreduced (2n) gametes from an interspecific hybrid of Brassica napus L. × Brassica carinata Braun

Nelson, Matthew N., Mason, Annaliese S., Castello, Marie-Claire, Thomson, Linda, Yan, Guijun and Cowling, Wallace A. (2009) Microspore culture preferentially selects unreduced (2n) gametes from an interspecific hybrid of Brassica napus L. × Brassica carinata Braun. Theoretical and Applied Genetics, 119 3: 497-505. doi:10.1007/s00122-009-1056-8


Author Nelson, Matthew N.
Mason, Annaliese S.
Castello, Marie-Claire
Thomson, Linda
Yan, Guijun
Cowling, Wallace A.
Title Microspore culture preferentially selects unreduced (2n) gametes from an interspecific hybrid of Brassica napus L. × Brassica carinata Braun
Formatted title
Microspore culture preferentially selects unreduced (2n) gametes from an interspecific hybrid of Brassica napus L. × Brassica carinata Braun
Journal name Theoretical and Applied Genetics   Check publisher's open access policy
ISSN 0040-5752
1432-2242
Publication date 2009-08-01
Sub-type Article (original research)
DOI 10.1007/s00122-009-1056-8
Volume 119
Issue 3
Start page 497
End page 505
Total pages 9
Place of publication Heidelberg, Germany
Publisher Springer
Language eng
Formatted abstract
We analysed the products of male meiosis in microspore-derived progeny from a Brassica napus (AACnCn) × Brassica carinata (BBCcCc) interspecific hybrid (ABCnCc). Genotyping at 102 microsatellite marker loci and nuclear DNA contents provided strong evidence that 26 of the 28 progeny (93%) were derived from unreduced (2n) gametes. The high level of CnCc marker heterozygosity, and parallel spindles at Anaphase II in the ABCnCc hybrid, indicated that unreduced gametes were formed by first division restitution. The frequency of dyads at the tetrad stage of pollen development (2.6%) suggested that unreduced gametes were preferentially selected in microspore culture. Segregation of marker alleles in the microspore-derived progeny was consistent with homologous recombination between Cn and Cc chromosomes and homoeologous recombination involving A-, B- and C-genome chromosomes during meiosis in the ABCnCc hybrid. We discuss the potential for using microspore culture of unreduced gametes in interspecific hybrids to map Brassica centromeres through half-tetrad analysis.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Agriculture and Food Sciences
 
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Created: Thu, 15 Mar 2012, 21:17:34 EST by Annaliese Mason on behalf of School of Agriculture and Food Sciences