Allowance for effects of thermodynamic nonideality in sedimentation equilibrium distributions reflecting protein dimerization

Wills, Peter R., Scott, David J. and Winzor, Donald J. (2011) Allowance for effects of thermodynamic nonideality in sedimentation equilibrium distributions reflecting protein dimerization. Analytical Biochemistry, 422 1: 28-32. doi:10.1016/j.ab.2011.12.010


Author Wills, Peter R.
Scott, David J.
Winzor, Donald J.
Title Allowance for effects of thermodynamic nonideality in sedimentation equilibrium distributions reflecting protein dimerization
Language of Title eng
Journal name Analytical Biochemistry   Check publisher's open access policy
Language of Journal Name eng
ISSN 0003-2697
1096-0309
Publication date 2011-12-12
Year available 2011
Sub-type Article (original research)
DOI 10.1016/j.ab.2011.12.010
Volume 422
Issue 1
Start page 28
End page 32
Total pages 5
Editor William Jakoby
Place of publication Maryland Heights, MO, United States
Publisher Academic Press
Collection year 2012
Language eng
Formatted abstract
This reexamination of a high-speed sedimentation equilibrium distribution for α-chymotrypsin under slightly acidic conditions (pH 4.1, IM 0.05) has provided experimental support for the adequacy of nearest-neighbor considerations in the allowance for effects of thermodynamic nonideality in the characterization of protein self-association over a moderate concentration range (up to 8 mg/mL). A widely held but previously untested notion about allowance for thermodynamic nonideality effects is thereby verified experimentally. However, it has also been shown that a greater obstacle to better characterization of protein self-association is likely to be the lack of a reliable estimate of monomer net charge, a parameter that has a far more profound effect on the magnitude of the measured equilibrium constant than any deficiency in current procedures for incorporating the effects of thermodynamic nonideality into the analysis of sedimentation equilibrium distributions reflecting reversible protein self-association.
Keyword Sedimentation equilibrium
Protein self-association
Thermodynamic nonideality
α-Chymotrypsin
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Article first published online December 2011. Not published in print until March 2012. Volume, issue and page numbers refer to print publication.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
School of Chemistry and Molecular Biosciences
 
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Created: Tue, 13 Mar 2012, 18:25:32 EST by Lucy O'Brien on behalf of School of Chemistry & Molecular Biosciences