Direct visualization of capsaicin and vanillylamine in Capsicum fruit with laser flurometric imaging

Suzuki, Hideo, Ohta, Mari, Watanabe, Tatsuo and Suzuki, Tetsuya (2011) Direct visualization of capsaicin and vanillylamine in Capsicum fruit with laser flurometric imaging. Trace Nutrients Research, 28: 35-39.

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Name Description MIMEType Size Downloads
Author Suzuki, Hideo
Ohta, Mari
Watanabe, Tatsuo
Suzuki, Tetsuya
Title Direct visualization of capsaicin and vanillylamine in Capsicum fruit with laser flurometric imaging
Language of Title eng
Journal name Trace Nutrients Research
Language of Journal Name eng
Publication date 2011
Sub-type Article (original research)
Issue 28
Start page 35
End page 39
Total pages 5
Place of publication Japan
Publisher Japan Trace Nutrients Research Society
Collection year 2012
Language eng
Abstract Capsaicin. the pungent secondary metabolite found in fruit of the genus Capsicum, and vanillylamine. a metabolic precursor to capsaicin. are used in the food science. pharmaceutical. medical. and forensic industries; however. a rapid method to evaluate fruit for the presence of these two compounds is lacking. The present research describes the in situ visualization of capsaicin and its precursor in Capsicum fruit by laser-induced fluorescence imaging and spectrometry. When excited by ultraviolet lasers at 266 nm. capsaicin and vanillylamine have peak fluorescence emissions at 310 nm. The fluorescence spectra of precursors of capsaicin and analogs, i.e., trans-caffeic acid, trans ferulic acid, trans-para-coumaric acid, vanillyl alcohol. vanillin, vanillic acid, had different peaks than those of capsaicin and vanillylamine. The localization of capsaicin and its immediate precursor. vanillylamine, was imaged with an ultraviolet-sensitive camera after excitation with a laser at 266 nm. Fluorescence images detected at 310 nm showed the localization of capsaicinoid and/ or vanillylamine on the surface of placenta and septa of Capsicum fruits. No fluorescence specific to capsaicin and vanillylamine was observed in seeds or pericarp. Both bell pepper and sweet pepper also showed 310 nm fluorescence on the placental surface, suggesting the accumulation of vanillylamine in the placenta. Laser-induced imaging shows considerable promise as a suitable technique for rapidly screening Capsicum fruit for their capsaicin and vanillylamine contents.
Q-Index Code CX
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Proceedings of the Symposium on Trace Nutrients Research organized by the Japan Trace Nutrients Research Society

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
National Research Centre for Environmental Toxicology Publications
 
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Created: Mon, 12 Mar 2012, 14:21:24 EST by Robyne Anderson on behalf of National Res Centre For Environmental Toxicology