In vitro regeneration and transformation of Blackstonia perfoliata

Bijelovic, A., Rosic, N., Miljus-Djukic, J., Ninkovic, S. and Grubisic, D. (2004) In vitro regeneration and transformation of Blackstonia perfoliata. Biologia Plantarum, 48 3: 333-338. doi:10.1023/B:BIOP.0000041083.41294.31


Author Bijelovic, A.
Rosic, N.
Miljus-Djukic, J.
Ninkovic, S.
Grubisic, D.
Title In vitro regeneration and transformation of Blackstonia perfoliata
Journal name Biologia Plantarum   Check publisher's open access policy
ISSN 0006-3134
1573-8264
Publication date 2004
Sub-type Article (original research)
DOI 10.1023/B:BIOP.0000041083.41294.31
Volume 48
Issue 3
Start page 333
End page 338
Total pages 6
Place of publication Dordrecht, Netherlands
Publisher Springer
Language eng
Formatted abstract
In vitro root culture of yellow wort (Blackstonia perfoliata (L.) Huds.) was initiated on Murashige and Skoog (MS) medium. In the presence of benzylaminopurine (BAP) numerous adventitious buds formed, which developed into shoots. Presence of indole-3-butyric acid (IBA) in media significantly decreased number of buds, but increased development of lateral roots. On hormone-free medium shoots successfully rooted and developed flowers and viable seeds that formed another generation. Shoot cultures of B. perfoliata inoculated with suspension of Agrobacterium rhizogenes strain A4M70GUS developed hairy roots at 3 weeks and they were cultured on hormone-free MS medium. Spontaneous shoot regeneration occurred in 3 clones.
Keyword Agrobacterium rhizogenes
Hairy roots
Regeneration
Root culture
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Biological Sciences Publications
 
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Created: Mon, 12 Mar 2012, 10:22:43 EST by Ms Nela Rosic on behalf of School of Biological Sciences