Secoiridoid content of Blackstonia perfoliata in vivo and in vitro

Sabovljevic, Aneta, Rosic, Nedeljka, Jankovic, Teodora and Grubisic, Dragoljub (2006) Secoiridoid content of Blackstonia perfoliata in vivo and in vitro. In Vitro Cellular and Developmental Biology: Plant, 42 5: 427-431. doi:10.1079/IVP2006759


Author Sabovljevic, Aneta
Rosic, Nedeljka
Jankovic, Teodora
Grubisic, Dragoljub
Title Secoiridoid content of Blackstonia perfoliata in vivo and in vitro
Formatted title
Secoiridoid content of Blackstonia perfoliata in vivo and in vitro
Journal name In Vitro Cellular and Developmental Biology: Plant   Check publisher's open access policy
ISSN 1054-5476
1475-2689
Publication date 2006-09
Sub-type Article (original research)
DOI 10.1079/IVP2006759
Volume 42
Issue 5
Start page 427
End page 431
Total pages 5
Place of publication Heidelberg, Germany
Publisher Springer
Language eng
Formatted abstract
This study reports the analysis of secondary metabolites of gentiopicrin, swertiamarin, and sweroside in shoot and root cultures of yellow wort (Blackstonia perfoliata), which were initiated from seeds, grown on Murashige and Skoog (MS) medium. Shoot cultures of B. perfoliata were inoculated with suspension of Agrobacterium rhizogenes strain A4M70GUS and hairy roots appeared at the infected sites after 3 wk of inoculation. Tips of adventitious roots of B. perfoliata were grown on hormone-free MS medium and three clones of the transformed roots regenerated shoots spontaneously. Gentiopicrin, swertiamarin, and sweroside were detected in both roots and shoots of B. perfoliata in vitro and in vivo, but gentiopicrin was found to be the major compound. The concentration of growth regulator in the medium affected the production of secoiridoids in B. perfoliata in vitro, where the level of gentiopicrin was higher in plants grown in the presence of indole-3-butyric acid, but the presence of 6-benzylaminopurine was inhibitory to secoiridoid production.
Keyword Gentiopicrin
Root and shoot culture
Sweroside
Swertiamarin
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Biological Sciences Publications
 
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Created: Mon, 12 Mar 2012, 10:19:59 EST by Ms Nela Rosic on behalf of School of Biological Sciences