A shorter interval between irradiation of recipient testis and germ cell transplantation is detrimental to recovery of fertility in rams

Herrid, M., Davey, R., Stockwell, S., Olejnik, J., Schmoelzl, S., Suchowerska, N., Jackson, M., Holland, M. and Hill, J.R. (2011) A shorter interval between irradiation of recipient testis and germ cell transplantation is detrimental to recovery of fertility in rams. International Journal of Andrology, 34 5 Part 1: 501-512. doi:10.1111/j.1365-2605.2010.01113.x


Author Herrid, M.
Davey, R.
Stockwell, S.
Olejnik, J.
Schmoelzl, S.
Suchowerska, N.
Jackson, M.
Holland, M.
Hill, J.R.
Title A shorter interval between irradiation of recipient testis and germ cell transplantation is detrimental to recovery of fertility in rams
Language of Title eng
Journal name International Journal of Andrology   Check publisher's open access policy
Language of Journal Name eng
ISSN 0105-6263
1365-2605
Publication date 2011-10
Year available 2010
Sub-type Article (original research)
DOI 10.1111/j.1365-2605.2010.01113.x
Volume 34
Issue 5 Part 1
Start page 501
End page 512
Total pages 12
Place of publication Oxford, United Kingdom
Publisher Wiley-Blackwell Publishing
Collection year 2012
Language eng
Abstract The objective of the current study was to identify an optimal time period for donor cell transplantation after irradiation in sheep. The testes of recipient rams were treated with a single dose of 15 Gray (Gy) irradiation followed by germ cell transplantation either 3 or 6weeks later. Transplantation of donor cells at 6weeks after irradiation resulted in production of donor sperm by all five recipient rams compared with 4 of 11 rams transplanted at 3weeks. Rams transplanted 3weeks post-irradiation appeared to show reduced libido and fertility. Two rams produced sperm with low motility (<20%) and two other rams were azoospermic. More than 1year after cell transfer, there were heavy infiltrates of CD45-positive cells and more fibrous tissue in 9 of 14 recipient testes (seven rams) that received cells 3weeks after irradiation. Taken together, these results suggest that the interval between irradiation of recipients and germ cell transplantation affects the success rate of the procedure, with a 6-week interval preferable. The elevated inflammatory/immune reaction may be responsible, at least in part, for the reduced fertility and low libido observed in the rams that received cells 3weeks post-irradiation.
Keyword Animal model
Sheep
Spermatogenesis
Testis
Testis germ cell transplantation
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
School of Veterinary Science Publications
 
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Created: Thu, 08 Mar 2012, 18:25:51 EST by Professor Jonathan Hill on behalf of School of Veterinary Science