The effective time of centrifugation for the analysis of boundary spreading in sedimentation velocity experiments

Besong, Tabot M. D., Harding, Stephen E. and Winzor, Donald J. (2012) The effective time of centrifugation for the analysis of boundary spreading in sedimentation velocity experiments. Analytical Biochemistry, 421 2: 755-758. doi:10.1016/j.ab.2011.11.035


Author Besong, Tabot M. D.
Harding, Stephen E.
Winzor, Donald J.
Title The effective time of centrifugation for the analysis of boundary spreading in sedimentation velocity experiments
Journal name Analytical Biochemistry   Check publisher's open access policy
ISSN 0003-2697
1096-0309
Publication date 2012-02-15
Year available 2011
Sub-type Article (original research)
DOI 10.1016/j.ab.2011.11.035
Volume 421
Issue 2
Start page 755
End page 758
Total pages 4
Place of publication Maryland Heights, MO, United States
Publisher Academic Press
Collection year 2012
Language eng
Formatted abstract
This investigation establishes a likely order of magnitude for the zero-time correction factor governing the effective time of centrifugation that is pertinent in the analysis of boundary spreading in sedimentation velocity experiments. This correction is shown to be too small to unduly affect the magnitudes of sedimentation and diffusion coefficients deduced from the application of computer software incorporating the printout value of ω2t and an effective position of the air–solution meniscus that is obtained as an additional parameter in the analysis involving nonlinear least-squares curve-fitting of sedimentation velocity distributions to the Lamm equation. Although this procedure slightly underestimates the actual meniscus position (ra), uncertainty about its exact location precludes adoption of the alternative approach with ra fixed and the correction to ω2t regarded as the additional curve-fitting parameter.
Keyword Sedimentation velocity
Effective time of centrifugation
Boundary spreading
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Article first published online 1 December 2011

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
School of Chemistry and Molecular Biosciences
 
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Created: Thu, 08 Mar 2012, 08:14:25 EST by Lucy O'Brien on behalf of School of Chemistry & Molecular Biosciences