Effects of medicinal herb salvia miltiorrhiza on osteoblastic cells in vitro

Chin, Alice, Yang, Yanqi, Chai, Lei, Wong, Ricky W. K. and Rabie, Abou-Bakr M. (2011) Effects of medicinal herb salvia miltiorrhiza on osteoblastic cells in vitro. Journal of Orthopaedic Research, 29 7: 1059-1063. doi:10.1002/jor.21376

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Author Chin, Alice
Yang, Yanqi
Chai, Lei
Wong, Ricky W. K.
Rabie, Abou-Bakr M.
Title Effects of medicinal herb salvia miltiorrhiza on osteoblastic cells in vitro
Language of Title eng
Journal name Journal of Orthopaedic Research   Check publisher's open access policy
Language of Journal Name eng
ISSN 0736-0266
Publication date 2011-07
Sub-type Article (original research)
DOI 10.1002/jor.21376
Volume 29
Issue 7
Start page 1059
End page 1063
Total pages 5
Place of publication Hoboken, NJ, United States
Publisher John Wiley & Sons
Collection year 2012
Language eng
Abstract Sufficient osteoinduction is essential for the success and effectiveness of bone grafting. It was previously found that Salvia Miltiorrhiza (SM), a commonly used Chinese herb increased osteogenesis in vivo. The aim of this study is to investigate the effects of SM on bone cells in vitro, in an attempt to get a better understanding on how SM can promote bone remodeling. MC3T3-E1, an osteoblastic cell line, was cultured with SM for different time intervals (24, 48, and 72 h), whereas the control group consisted of cells cultured without any intervention. The mRNA expression of alkaline phosphatase (ALP), osteocalcin (OCN), osteoprotegerin (OPG), and the receptor activator of nuclear factor kappa B ligand (RANKL) were examined by real-time polymerase chain reaction (qPCR). The expression of ALP showed an early increase at 24 h by 50% (p < 0.001) and at 48 h by 13% (p < 0.001). OCN was decreased by 22% at 24 h (p < 0.001) but increased by 50% and 88% at 48 and 72 h, respectively (p < 0.001). RANKL showed an early increase at the first two time points of 24 and 48 h by 45% (p < 0.001) and 36% (p < 0.01), respectively, while OPG was up-regulated at the latter two time points by 10% at 48 h (p < 0.01) and 68% at 72 h (p < 0.001). Thus, OPG/RANKL was down-regulated first, and then up-regulated. SM enhances bone remodeling by regulating the gene expression of ALP, OCN, OPG, and RANKL. It is a potential medicinal herb to be utilized in the application that requires stimulation in bone cell activities.
Keyword Salvia miltiorrhiza
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
School of Dentistry Publications
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Created: Wed, 07 Mar 2012, 14:15:42 EST by Dr Lei Chai on behalf of School of Dentistry