Conjugation of immunoglobulin M to gold nanoparticles

Wang, Hui and Dimitrov, Krassen (2011) Conjugation of immunoglobulin M to gold nanoparticles. Current Nanoscience, 7 6: 874-878. doi:10.2174/157341311798220682

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Author Wang, Hui
Dimitrov, Krassen
Title Conjugation of immunoglobulin M to gold nanoparticles
Journal name Current Nanoscience   Check publisher's open access policy
ISSN 1573-4137
Publication date 2011-12
Sub-type Article (original research)
DOI 10.2174/157341311798220682
Volume 7
Issue 6
Start page 874
End page 878
Total pages 5
Place of publication Bussum, Netherlands
Publisher Bentham Science Publishers
Collection year 2012
Language eng
Abstract Due to unique optical properties, facile bioconjugation, and biocompatibility, colloidal gold nanoparticles have been widely used for nuclear targeting, gene and drug delivery, and biomedical diagnosis and therapeutics. Various peptides, proteins, and polymers, etc. have been utilized to functionalize gold nanoparticles. Especially antibodies-AuNPs conjugates have drawn more and more attractions due to their specific affinities to the target antigens. However, to our best knowledge, immunoglobulin M (IgM) has not been applied for AuNPs modification so far. In this work, we present a simple modification process to immobilize IgM on the surface of gold nanoparticles. Dark field microscopy, light scattering, and colorimetric assays were conducted to monitor the conjugation process. The properties of AuNPs before and after IgM modification were further studied. The results demonstrated that the stability of AuNPs was improved upon IgM conjugation. This study may open a new way to apply IgM conjugated AuNPs for biomedical potential applications.
Keyword Colorimetric assay
Dark field microscopy
Gold nanoparticles
Immunoglobulin M
Light scattering
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
Australian Institute for Bioengineering and Nanotechnology Publications
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Citation counts: TR Web of Science Citation Count  Cited 2 times in Thomson Reuters Web of Science Article | Citations
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