Immunological response to parenteral vaccination with recombinant hepatitis B virus surface antigen virus-like particles expressing Helicobacter pylori KatA epitopes in a murine H. pylori challenge model

Kotiw, Michael, Johnson, Megan, Pandey, Manisha, Fry, Scott, Hazell, Stuart L., Netter, Hans J., Good, Michael F. and Olive, Colleen (2012) Immunological response to parenteral vaccination with recombinant hepatitis B virus surface antigen virus-like particles expressing Helicobacter pylori KatA epitopes in a murine H. pylori challenge model. Clinical and Vaccine Immunology, 19 2: 268-276. doi:10.1128/CVI.05295-11


Author Kotiw, Michael
Johnson, Megan
Pandey, Manisha
Fry, Scott
Hazell, Stuart L.
Netter, Hans J.
Good, Michael F.
Olive, Colleen
Title Immunological response to parenteral vaccination with recombinant hepatitis B virus surface antigen virus-like particles expressing Helicobacter pylori KatA epitopes in a murine H. pylori challenge model
Formatted title
Immunological response to parenteral vaccination with recombinant hepatitis B virus surface antigen virus-like particles expressing Helicobacter pylori KatA epitopes in a murine H. pylori challenge model
Journal name Clinical and Vaccine Immunology   Check publisher's open access policy
ISSN 1556-6811
1095-8282
Publication date 2012-02-01
Year available 2011
Sub-type Article (original research)
DOI 10.1128/CVI.05295-11
Volume 19
Issue 2
Start page 268
End page 276
Total pages 9
Place of publication Oxford, United Kingdom
Publisher Elsevier
Collection year 2012
Language eng
Formatted abstract
Virus-like particles (VLPs) based on the small envelope protein of hepatitis B virus (HBsAg-S) are immunogenic at the B- and T-cell level. In this study, we inserted overlapping sequences encoding the carboxy terminus of the Helicobacter pylori katA gene product into HBsAg-S. The HBsAg-S–KatA fusion proteins were able to assemble into secretion-competent VLPs (VLP-KatA). The VLP-KatA proteins were able to induce KatA-specific antibodies in immunized mice. The mean total IgG antibody titers 41 days post-primary immunization with VLP-KatA (2.3 × 103) were significantly greater (P < 0.05) than those observed for vaccination with VLP alone (5.2 × 102). Measurement of IgG isotypes revealed responses to both IgG1 and IgG2a (mean titers, 9.0 × 104 and 2.6 × 104, respectively), with the IgG2a response to vaccination with VLP-KatA being significantly higher than that for mice immunized with KatA alone (P < 0.05). Following challenge of mice with H. pylori, a significantly reduced bacterial load in the gastric mucosa was observed (P < 0.05). This is the first report describing the use of VLPs as a delivery vehicle for H. pylori antigens.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published ahead of print 28 December 2011

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
Institute for Molecular Bioscience - Publications
 
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