Digital hypothermia inhibits early lamellar inflammatory signalling in the oligofructose laminitis model

van Eps, A. W., Leise, B. S., Watts, M., Pollitt, C. C. and Belknap, J. K. (2012) Digital hypothermia inhibits early lamellar inflammatory signalling in the oligofructose laminitis model. Equine Veterinary Journal, 44 2: 230-237. doi:10.1111/j.2042-3306.2011.00416.x


Author van Eps, A. W.
Leise, B. S.
Watts, M.
Pollitt, C. C.
Belknap, J. K.
Title Digital hypothermia inhibits early lamellar inflammatory signalling in the oligofructose laminitis model
Journal name Equine Veterinary Journal   Check publisher's open access policy
ISSN 0425-1644
2042-3306
Publication date 2012-03
Year available 2011
Sub-type Article (original research)
DOI 10.1111/j.2042-3306.2011.00416.x
Volume 44
Issue 2
Start page 230
End page 237
Total pages 8
Place of publication Hoboken, NJ, U.S.A.
Publisher John Wiley & Sons
Collection year 2013
Language eng
Formatted abstract
Reasons for performing study: The pathophysiological events inhibited by prophylactic digital hypothermia that result in reduction of the severity of acute laminitis are unknown.

Objectives: To determine if digital hypothermia inhibits lamellar inflammatory signalling during development of oligofructose (OF) induced laminitis.

Methods: Fourteen Standardbred horses were given 10 g/kg bwt OF by nasogastric tube with one forelimb (CRYO) continuously cooled by immersion in ice and water and one forelimb (NON-RX) at ambient temperature. Lamellae were harvested prior to the onset of lameness (24 h post OF administration, DEV group, n = 7) or at the onset of lameness (OG1 group, n = 7). Lamellar mRNA was purified and cDNA produced for real time-quantitative PCR analysis of mRNA concentrations of cytokines (IL-6, IL-1β, IL-10), chemokines (CXCL1, CXCL6, CXCL8/IL-8, MCP-1, MCP-2), cell adhesion molecules (ICAM-1, E-selectin), COX-2 and 3 housekeeping genes. Data were analysed (NON-RX vs. CRYO, NON-RX vs. archived control [CON, n = 7] lamellar tissue) using nonparametric tests.

Results: Compared with CON, the OG1 NON-RX had increased (P<0.05) lamellar mRNA concentrations of all measured mediators except IL-10, IL-1β and MCP-1/2, whereas only CXCL8 was increased (P<0.05) in DEV NON-RX. Within the OG1 group, CRYO limbs (compared with NON-RX) had decreased (P<0.05) mRNA concentrations of the majority of measured inflammatory mediators (no change in MCP-1 and IL-10). Within the DEV group, mRNA concentrations of CXCL-1, ICAM-1, IL-1β, CXCL8 and MCP-2 were decreased (P<0.05) and the anti-inflammatory cytokine IL-10 was increased (compared with NON-RX limbs; P<0.05).

Conclusions: Digital hypothermia effectively blocked early lamellar inflammatory events likely to play an important role in lamellar injury including the expression of chemokines, proinflammatory cytokines, COX-2 and endothelial adhesion molecules.

Potential relevance: This study demonstrates a potential mechanism by which hypothermia reduces the severity of acute laminitis, and may help identify molecular targets for future laminitis intervention.
Keyword Horse
Laminitis
Hypothermia
Cryotherapy
Inflammation
Cytokines
Walnut-induced laminitis
Nf-kappa-B
Equine laminitis
Prodromal stage
Adhesion molecule-1
Gene-expression
Nitric-oxide
Lung injury
In-vivo
Horses
NF-κB
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Article first published online: 5 SEP 2011

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
School of Veterinary Science Publications
 
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