Evaluation of BMP-2 gene-activated muscle grafts for cranial defect repair.

Liu, Fangjun, Porter, Ryan M., Wells, James, Glatt, Vaida, Pilapil, Carmencita and Evans, Christopher H. (2011) Evaluation of BMP-2 gene-activated muscle grafts for cranial defect repair.. Journal of Orthopaedic Research, 30 7: 1095-1102. doi:10.1002/jor.22038

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Author Liu, Fangjun
Porter, Ryan M.
Wells, James
Glatt, Vaida
Pilapil, Carmencita
Evans, Christopher H.
Title Evaluation of BMP-2 gene-activated muscle grafts for cranial defect repair.
Journal name Journal of Orthopaedic Research   Check publisher's open access policy
ISSN 0736-0266
Publication date 2011-12-28
Year available 2011
Sub-type Article (original research)
DOI 10.1002/jor.22038
Volume 30
Issue 7
Start page 1095
End page 1102
Total pages 8
Place of publication Hoboken, NJ, U.S.A.
Publisher John Wiley & Sons
Collection year 2012
Language eng
Formatted abstract
Large, osseous, segmental defects heal poorly. Muscle has a propensity to form bone when exposed to an osteogenic stimulus such as that provided by transfer and expression of cDNA encoding bone morphogenetic protein-2 (BMP-2). The present study evaluated the ability of genetically modified, autologous muscle to heal large cranial defects in rats. Autologous grafts (8 mm × 2 mm) were punched from the biceps femoris muscle and transduced intraoperatively with recombinant adenovirus vector containing human BMP-2 or green fluorescent protein cDNA. While the muscle biopsies were incubating with the vector, a central parietal 8 mm defect was surgically created in the calvarium of the same animal. The gene-activated muscle graft was then implanted into the cranial defect. After 8 weeks, crania were examined radiographically, histologically, and by micro-computed tomography and dual energy X-ray absorptiometry. Although none of the defects were completely healed in this time, muscle grafts expressing BMP-2 deposited more than twice as much new bone as controls. Histology confirmed the anatomical integrity of the newly formed bone, which was comparable in thickness and mineral density to the original cranial bone. This study confirms the in vivo osteogenic properties of genetically modified muscle and suggests novel strategies for healing bone.
Keyword Gene therapy
Bone healing
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status Non-UQ
Additional Notes Article first published online: 28 DEC 2011.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
UQ Diamantina Institute Publications
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Citation counts: TR Web of Science Citation Count  Cited 16 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 15 times in Scopus Article | Citations
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Created: Thu, 01 Mar 2012, 15:59:59 EST by Dr James Wells on behalf of UQ Diamantina Institute