Expression of GFAP splice variants in the rodent central nervous system

Sullivan, S. M., Sullivan, R. K. P., Yong, K. and Colditz, P. B. (2012). Expression of GFAP splice variants in the rodent central nervous system. In: Australian Neuroscience Society Meeting, Gold Coast, Australia, (). 30 January - 1 February 2012.

Author Sullivan, S. M.
Sullivan, R. K. P.
Yong, K.
Colditz, P. B.
Title of paper Expression of GFAP splice variants in the rodent central nervous system
Language of Title eng
Conference name Australian Neuroscience Society Meeting
Conference location Gold Coast, Australia
Conference dates 30 January - 1 February 2012
Language of Proceedings Title eng
Language of Journal Name eng
Publication Year 2012
Sub-type Poster
Language eng
Formatted Abstract/Summary
Purpose: Astrocytes can be identified in the brain by the expression of glial fibrillary acidic protein (GFAP). GFAP is expressed in the core or cytoskeleton of the astrocyte and its function is to provide stability to the cell processes. The expression of GFAP is altered in many diseases that affect the central nervous system (CNS), including multiple sclerosis, stroke, Alzheimer’s disease and traumatic brain injury. Novel isoforms of GFAP (GFAPε and GFAPκ) have recently been identified and may play an important role in development or disease. GFAPε and GFAPκ differ from GFAPα in the C’ terminal region of the protein and have been associated with destabilising the formation of GFAP polymers.
Methods: We have generated polyclonal antibodies against GFAPα, GFAPε and GFAPκ and examined the expression of GFAP splice variants using western blotting and immunohistochemistry in CNS tissues from rats, mice and guinea pigs (N=9).
Results: Immunohistochemistry revealed that GFAP splice variants co-localized with regular GFAP, detected using a commercial polyclonal antibody from DakoCytomation. GFAPα, GFAPε and GFAPκ were detectable by western blotting in the majority of tissues examined (cortex, hippocampus, thalamus, brain stem, cerebellum, spinal cord and retina). Western blotting revealed slightly different expression profiles of the splice variants, with GFAPκ weakly expressed in the retinas of rats, mice and guinea pigs and GFAPε strongly expressed in guinea pig retina.
Conclusion: This study is the first to demonstrate the protein expression of GFAP splice variants. Future studies will examine what role these splice variants might play in development and disease.
Q-Index Code EX
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Conference Paper
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Created: Thu, 01 Mar 2012, 13:26:48 EST by Dr Susan Sullivan on behalf of UQ Centre for Clinical Research