Review of fluorescent standards for calibration of in situ fluorometers: Recommendations applied in coastal and ocean observing programs

Earp, Alan, Hanson, Christine E., Ralph, Peter J., Brando, Vittorio E., Allen, Simon, Baird, Mark, Clementson, Lesley, Daniel, Paul, Dekker, Arnold G., Fearns, Peter R. C. S., Parslow, John, Strutton, Peter G., Thompson, Peter A., Underwood, Mark, Weeks, Scarla and Doblin, Martina A. (2011) Review of fluorescent standards for calibration of in situ fluorometers: Recommendations applied in coastal and ocean observing programs. Optics Express, 19 27: 26768-26782. doi:10.1364/OE.19.026768


Author Earp, Alan
Hanson, Christine E.
Ralph, Peter J.
Brando, Vittorio E.
Allen, Simon
Baird, Mark
Clementson, Lesley
Daniel, Paul
Dekker, Arnold G.
Fearns, Peter R. C. S.
Parslow, John
Strutton, Peter G.
Thompson, Peter A.
Underwood, Mark
Weeks, Scarla
Doblin, Martina A.
Title Review of fluorescent standards for calibration of in situ fluorometers: Recommendations applied in coastal and ocean observing programs
Formatted title
Review of fluorescent standards for calibration of in situ fluorometers: Recommendations applied in coastal and ocean observing programs
Journal name Optics Express   Check publisher's open access policy
ISSN 1094-4087
Publication date 2011-12-19
Sub-type Article (original research)
DOI 10.1364/OE.19.026768
Open Access Status DOI
Volume 19
Issue 27
Start page 26768
End page 26782
Total pages 15
Place of publication Washington, DC, U.S.A.
Publisher Optical Society of America
Collection year 2012
Language eng
Formatted abstract
Fluorometers are widely used in ecosystem observing to monitor fluorescence signals from organic compounds, as well as to infer geophysical parameters such as chlorophyll or CDOM concentration, but measurements are susceptible to variation caused by biofouling, instrument design, sensor drift, operating environment, and calibration rigor. To collect high quality data, such sensors need frequent checking and regular calibration. In this study, a wide variety of both liquid and solid fluorescent materials were trialed to assess their suitability as reference standards for performance assessment of in situ fluorometers. Criteria used to evaluate the standards included the spectral excitation/emission responses of the materials relative to fluorescence sensors and to targeted ocean properties, the linearity of the fluorometer’s optical response with increasing concentration, stability and consistency, availability and ease of use, as well as cost. Findings are summarized as a series of recommended reference standards for sensors deployed on stationary and mobile platforms, to suit a variety of in situ coastal to ocean sensor configurations. Repeated determinations of chlorophyll scale factor using the recommended liquid standard, Fluorescein, achieved an accuracy of 2.5%. Repeated measurements with the recommended solid standard, Plexiglas Satinice® plum 4H01 DC (polymethylmethacrylate), over an 18 day period varied from the mean value by 1.0% for chlorophyll sensors and 3.3% for CDOM sensors.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Geography, Planning and Environmental Management Publications
Official 2012 Collection
 
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Created: Mon, 27 Feb 2012, 14:43:14 EST by Alexandra Simmonds on behalf of School of Geography, Planning & Env Management