The timeline of metalloprotease events during oligofructose induced equine laminitis development

Visser, M.B. and Pollitt, C.C. (2012) The timeline of metalloprotease events during oligofructose induced equine laminitis development. Equine Veterinary Journal, 44 1: 88-93. doi:10.1111/j.2042-3306.2011.00393.x


Author Visser, M.B.
Pollitt, C.C.
Title The timeline of metalloprotease events during oligofructose induced equine laminitis development
Journal name Equine Veterinary Journal   Check publisher's open access policy
ISSN 0425-1644
2042-3306
Publication date 2012-01
Year available 2011
Sub-type Article (original research)
DOI 10.1111/j.2042-3306.2011.00393.x
Volume 44
Issue 1
Start page 88
End page 93
Total pages 6
Place of publication Hoboken, N, United States
Publisher John Wiley & Sons
Collection year 2012
Language eng
Formatted abstract
Reason for performing study: The role of matrix metalloproteases (MMPs) and the timeline of proteolysis during laminitis development are incompletely understood.

Objectives: To determine the temporal progression of selected MMPs and protease regulators during laminitis development.

Methods: Five clinically normal Standardbred horses received, via nasogastric intubation, an oligofructose (OF) bolus (10g/kg bwt). Laminitis induction proceeded for 48h followed by euthanasia. Lamellar biopsies were obtained prior to dosing and at intervals during the treatment period for analysis (12, 18, 24, 30 and 36h and at 48h following euthanasia). Tissue samples were analysed by real-time PCR, zymography and western blotting.

Results: Activation of proMMP-2 occurs either simultaneously or at least 12h following lamellar basement membrane (BM) damage, while no activation of proMMP-9 is seen during OF laminitis induction. Aggrecanase gene expression increased initially at 12-18h post OF dosing, similar to BM changes. Gene expression of TIMP-2, a MMP regulator, decreases during laminitis development.

Conclusions: The MMP-2/MT1-MMP complex may not play a major role in initiating lamellar BM damage. Aggrecanase and TIMP-2 gene expression appear related to BM lamellar changes.

Potential relevance: MMPs, historically thought to cause laminitis, do not appear to play an initiating role in the lamellar lesion. Other host derived proteases and degradation of alternative lamellar matrix components need to be considered.
Keyword Horse
Laminitis
Matrix metalloprotease
ADAMTS
TIMP
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes published online: 23 June 2011.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
School of Veterinary Science Publications
 
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