Developing robust faecal near infrared spectroscopy calibrations to predict diet dry matter digestibility in cattle consuming tropical forages

Coates, D. B. and Dixon, R. M. (2011) Developing robust faecal near infrared spectroscopy calibrations to predict diet dry matter digestibility in cattle consuming tropical forages. Journal of Near Infrared Spectroscopy, 19 6: 507-519. doi:10.1255/jnirs.967

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Author Coates, D. B.
Dixon, R. M.
Title Developing robust faecal near infrared spectroscopy calibrations to predict diet dry matter digestibility in cattle consuming tropical forages
Journal name Journal of Near Infrared Spectroscopy   Check publisher's open access policy
ISSN 0967-0335
1751-6552
1364-6575
Publication date 2011
Sub-type Article (original research)
DOI 10.1255/jnirs.967
Volume 19
Issue 6
Start page 507
End page 519
Total pages 13
Place of publication W Sussex, United Kingdom
Publisher N I R Publications
Collection year 2012
Language eng
Formatted abstract Various studies, mainly from temperate areas, have reported calibrations developed from the near infrared (NIR) spectra of faeces (F.NIRS) for predicting diet digestibility in ruminants and there has been substantial variation in predictive accuracy as indicated by calibration and validation statistics. The present study was conducted to develop and examine the reliability and robustness of F.NIRS calibration equations to estimate dry matter digestibility (DMD) of forage diets ingested by cattle grazing in the rangelands of northern Australia. A large and diverse calibration data set of matched diet-faecal pairs was obtained over 10 years using three sampling methods: (1) grazed pasture with diet samples collected from oesophageal fistulated steers and faeces collected from resident cattle; (2) in vivo digestibility experiments with penned cattle fed forage hays; and (3) penned cattle fed pasture freshly harvested from the field. Estimated in vivo DMD reference values were determined using pepsin-cellulase in vitro analysis of diet samples. The final calibration set of 1052 samples represented 264 diets with DMD ranging from 38% to 75%. Calibration statistics for DMD% were: standard error of calibration = 1.87, standard error of cross validation = 1.91, and the coefficient of determination, r2 = 0.90. Factors of particular importance, with regard to the accuracy of DMD reference values, are identified and discussed and recommendations made for minimising reference errors. A comprehensive series of independent validation tests was conducted by selecting validation sample sets from the entire sample set according to a range of criteria. Each validation sub-set was tested using the calibration calculated from the remainder of the sample set. These tests showed that sampling method and experimental site often had important effects on calibration statistics and performance and also that the standard error of performance of the overall calibration would likely be <2.5 DMD percentage units when applied to samples sourced from regions and pasture types represented in the calibration. Despite the large size and diversity of the calibration data set it was concluded that robustness would likely be improved by expansion of the calibration data set.
Keyword Faecal NIRS
Grazing cattle
Digestibility
Robustness
Miss-match error
Validation
Northern Australia
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
Queensland Alliance for Agriculture and Food Innovation
 
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