Redistribution of caveolae during mitosis

Boucrot, Emmanuel, Howes, Mark T., Kirchhausen, Tomas and Parton, Robert G. (2011) Redistribution of caveolae during mitosis. Journal of Cell Science, 124 12: 1965-1972. doi:10.1242/jcs.076570

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Author Boucrot, Emmanuel
Howes, Mark T.
Kirchhausen, Tomas
Parton, Robert G.
Title Redistribution of caveolae during mitosis
Journal name Journal of Cell Science   Check publisher's open access policy
ISSN 1477-9137
Publication date 2011-06-15
Sub-type Article (original research)
DOI 10.1242/jcs.076570
Open Access Status File (Publisher version)
Volume 124
Issue 12
Start page 1965
End page 1972
Total pages 8
Place of publication Cambridge, United Kingdom
Publisher The Company of Biologists
Collection year 2012
Language eng
Abstract Caveolae form a specialized platform within the plasma membrane that is crucial for an array of important biological functions, ranging from signaling to endocytosis. Using total internal reflection fluorescence (TIRF) and 3D fast spinning-disk confocal imaging to follow caveola dynamics for extended periods, and electron microscopy to obtain high resolution snapshots, we found that the vast majority of caveolae are dynamic with lifetimes ranging from a few seconds to several minutes. Use of these methods revealed a change in the dynamics and localization of caveolae during mitosis. During interphase, the equilibrium between the arrival and departure of caveolae from the cell surface maintains the steady-state distribution of caveolin-1 (Cav1) at the plasma membrane. During mitosis, increased dynamics coupled to an imbalance between the arrival and departure of caveolae from the cell surface induces a redistribution of Cav1 from the plasma membrane to intracellular compartments. These changes are reversed during cytokinesis. The observed redistribution of Cav1 was reproduced by treatment of interphase cells with nocodazole, suggesting that microtubule rearrangements during mitosis can mediate caveolin relocalization. This study provides new insights into the dynamics of caveolae and highlights precise regulation of caveola budding and recycling during mitosis.
Keyword Caveolae
Cell cycle
Total internal reflection fluorescence
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
Institute for Molecular Bioscience - Publications
Centre for Microscopy and Microanalysis Publications
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Citation counts: TR Web of Science Citation Count  Cited 32 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 30 times in Scopus Article | Citations
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Created: Fri, 24 Feb 2012, 15:44:05 EST by Susan Allen on behalf of Institute for Molecular Bioscience