A Neutrophil-Derived Antiviral Protein - Induction-Requirements and Biological Properties

Ohmann, H.Bielefeldt, Campos, M., Fitzpatrick, D.R., Rapin, N. and Babiuk, L.A. (1989) A Neutrophil-Derived Antiviral Protein - Induction-Requirements and Biological Properties. Journal of Virology, 63 5: 1916-1923.

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Author Ohmann, H.Bielefeldt
Campos, M.
Fitzpatrick, D.R.
Rapin, N.
Babiuk, L.A.
Title A Neutrophil-Derived Antiviral Protein - Induction-Requirements and Biological Properties
Journal name Journal of Virology   Check publisher's open access policy
ISSN 0022-538X
Publication date 1989-05
Year available 1989
Sub-type Article (original research)
Open Access Status File (Publisher version)
Volume 63
Issue 5
Start page 1916
End page 1923
Total pages 8
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Language eng
Abstract Polymorphonuclear neutrophilic granulocytes (PMN) have been implicated as playing a role in antiviral defense. In addition to having phagocytic and cytotoxic activities, PMN may produce an antiviral substance with interferon (IFN)-like activity. The product, for which the name polyferon (PF) has been coined, is produced upon direct encounter of PMN with bovine herpesvirus 1 (BHV-1)-infected bovine cells or membranes thereof. Exposure to purified virus only does not induce PF. The intimate interaction between PMN and the membranes was also revealed by electron microscopy studies. Bovine cells infected with herpes simplex virus type 1 could also induce PF production by bovine PMN, whereas cells infected with BHV-2, herpes simplex virus type 2, equine herpesvirus 1, bovine respiratory syncytial virus, bovine viral diarrhea virus, or parainfluenza virus 3 were unable to do so. Results obtained in experiments using transfected cells expressing BHV-1 glycoproteins as well as blocking experiments using BHV-1 glycoprotein-monospecific antibodies suggested that a combination of both viral product(s) and host cell factor(s) unique to bovine cells is required for induction of PF production by PMN. PF, which appeared in detectable amounts 12 to 18 h after exposure of PMN to the appropriate inducer, could not be neutralized by antibodies to bovine IFN-alpha, -beta, and -gamma. PF may nevertheless belong to the IFN family of proteins, as indicated by its ability to induce 2',5'-oligoadenyl synthetase in various cell types that are responsive to bovine IFNs and by its antiviral spectrum. It does, however, differ from the other cytokines in most immunological characteristics tested so far, including major histocompatibility complex class II antigen induction, cell migration, and cytotoxicity.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

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Sub-type: Article (original research)
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