Macaque proteome response to highly pathogenic avian influenza and 1918 reassortant influenza virus infections

Brown, Joseph N., Palermo, Robert E., Baskin, Carole R., Gritsenko, Marina, Sabourin, Patrick J., Long, James P., Sabourin, Carol L., Bielefeldt-Ohmann, Helle, Garcia-Sastre, Adolfo, Albrecht, Randy, Tumpey, Terrence M., Jacobs, Jon M., Smith, Richard D. and Katze, Michael G (2010) Macaque proteome response to highly pathogenic avian influenza and 1918 reassortant influenza virus infections. Journal of Virology, 84 22: 12058-12068. doi:10.1128/JVI.01129-10


Author Brown, Joseph N.
Palermo, Robert E.
Baskin, Carole R.
Gritsenko, Marina
Sabourin, Patrick J.
Long, James P.
Sabourin, Carol L.
Bielefeldt-Ohmann, Helle
Garcia-Sastre, Adolfo
Albrecht, Randy
Tumpey, Terrence M.
Jacobs, Jon M.
Smith, Richard D.
Katze, Michael G
Title Macaque proteome response to highly pathogenic avian influenza and 1918 reassortant influenza virus infections
Journal name Journal of Virology   Check publisher's open access policy
ISSN 0022-538X
1098-5514
Publication date 2010-11
Year available 2010
Sub-type Article (original research)
DOI 10.1128/JVI.01129-10
Open Access Status DOI
Volume 84
Issue 22
Start page 12058
End page 12068
Total pages 11
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Language eng
Abstract The host proteome response and molecular mechanisms that drive disease in vivo during infection by a human isolate of the highly pathogenic avian influenza virus (HPAI) and 1918 pandemic influenza virus remain poorly understood. This study presents a comprehensive characterization of the proteome response in cynomolgus macaque (Macaca fascicularis) lung tissue over 7 days of infection with HPAI (the most virulent), a reassortant virus containing 1918 hemagglutinin and neuraminidase surface proteins (intermediate virulence), or a human seasonal strain (least virulent). A high-sensitivity two-dimensional liquid chromatographytandem mass spectroscopy strategy and functional network analysis were implemented to gain insight into response pathways activated in macaques during influenza virus infection. A macaque protein database was assembled and used in the identification of 35,239 unique peptide sequences corresponding to approximately 4,259 proteins. Quantitative analysis identified an increase in expression of 400 proteins during viral infection. The abundance levels of a subset of these 400 proteins produced strong correlations with disease progression observed in the macaques, distinguishing a "core" response to viral infection from a "high" response specific to severe disease. Proteome expression profiles revealed distinct temporal response kinetics between viral strains, with HPAI inducing the most rapid response. While proteins involved in the immune response, metabolism, and transport were increased rapidly in the lung by HPAI, the other viruses produced a delayed response, characterized by an increase in proteins involved in oxidative phosphorylation, RNA processing, and translation. Proteomic results were integrated with previous genomic and pathological analysis to characterize the dynamic nature of the influenza virus infection process.
Keyword Tandem Mass-Spectrometry
Epithelial-Cells
Transmission Efficiency
Functional Genomics
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: ERA 2012 Admin Only
School of Veterinary Science Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 17 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 20 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Fri, 24 Feb 2012, 14:36:26 EST by System User on behalf of School of Veterinary Science