Expression of the rubber-like protein, resilin, in developing and functional insect cuticle determined using a Drosophila anti-rec 1 resilin antibody

Wong, Darren C. C., Pearson, Roger D., Elvin, Christopher M. and Merritt, David J. (2012) Expression of the rubber-like protein, resilin, in developing and functional insect cuticle determined using a Drosophila anti-rec 1 resilin antibody. Developmental Dynamics, 241 2: 333-339. doi:10.1002/dvdy.23724


Author Wong, Darren C. C.
Pearson, Roger D.
Elvin, Christopher M.
Merritt, David J.
Title Expression of the rubber-like protein, resilin, in developing and functional insect cuticle determined using a Drosophila anti-rec 1 resilin antibody
Formatted title
Expression of the rubber-like protein, resilin, in developing and functional insect cuticle determined using a Drosophila anti-rec 1 resilin antibody
Journal name Developmental Dynamics   Check publisher's open access policy
ISSN 1058-8388
1097-0177
Publication date 2012-02
Sub-type Article (original research)
DOI 10.1002/dvdy.23724
Volume 241
Issue 2
Start page 333
End page 339
Total pages 7
Place of publication Hoboken, NJ, United States
Publisher John Wiley & Sons
Collection year 2013
Language eng
Formatted abstract
Background: The natural elastomeric protein, insect resilin, is the most efficient elastic material known, used to store energy for jumping and flight in a variety of insects. Here, an antibody to recombinant Drosophila melanogaster pro-resilin is used to examine resilin expression in Drosophila and a wider range of insects. Results: Immunostaining of Drosophila embryos reveals anti-resilin reactivity in epidermal patches that exhibit a dynamic spatial and temporal expression through late embryogenesis. Resilin is also detected in stretch receptors in the embryo. In developing adult Drosophila, resilin pads are described at the base of wings and at the base of flexible sensory hairs in pupae. Resilin is also detected in embryos of the tephritid fruitfly, Bactrocera tryoni, and two well-known concentrations of insect resilin: the flight muscle tendon of the dragonfly and the pleural arch of the flea. Conclusions: The anti-Rec1 antibody antibody developed using Drosophila pro-resilin as antigen is cross-reactive and is useful for detection of resilin in diverse insects. For the first time, resilin expression has been detected during embryogenesis, revealing segmental patches of resilin in the developing epidermis of Drosophila.
Keyword Flea
Dragonfly
Embryo
Pupa
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published under Special Issue Patterns & Phenotypes

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
School of Biological Sciences Publications
 
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