A comparison of individual and combined L-phenylalanine ammonia lyase and cationic peroxidase transgenes for engineering resistance in tobacco to necrotrophic pathogens

Way, Heather M., Birch, Robert G. and Manners, John M. (2011) A comparison of individual and combined L-phenylalanine ammonia lyase and cationic peroxidase transgenes for engineering resistance in tobacco to necrotrophic pathogens. Plant Biotechnology Reports, 5 4: 301-308.

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Author Way, Heather M.
Birch, Robert G.
Manners, John M.
Title A comparison of individual and combined L-phenylalanine ammonia lyase and cationic peroxidase transgenes for engineering resistance in tobacco to necrotrophic pathogens
Journal name Plant Biotechnology Reports
Publication date 2011-10
Sub-type Article
DOI 10.1007/s11816-011-0183-2
Volume number 5
Issue number 4
ISSN 1863-5466; 1863-5474
Start page 301
End page 308
Total pages 8
Place of publication Tokyo, Japan
Publisher Springer Japan
Collection year 2012
Language eng
Formatted abstract This study tested the relative and combined efficacy of ShPx2 and ShPAL transgenes by comparing Nicotianatabacum hybrids with enhanced levels of l-phenylalanine ammonia lyase (PAL) activity and cationic peroxidase (Prx) activity with transgenic parental lines that overexpress either transgene. The PAL/Prx hybrids expressed both transgenes driven by the 35S CaMV promoter, and leaf PAL and Prx enzyme activities were similar to those of the relevant transgenic parent and seven- to tenfold higher than nontransgenic controls. Lignin levels in the PAL/Prx hybrids were higher than the PAL parent and nontransgenic controls, but not significantly higher than the Prx parent. All transgenic plants showed increased resistance to the necrotrophs Phytophthora parasitica pv. nicotianae and Cercospora nicotianae compared to nontransgenic controls, with a preponderance of smaller lesion categories produced in Prx-expressing lines. However, the PAL/Prx hybrids showed no significant increase in resistance to either pathogen relative to the Prx parental line. These data indicate that, in tobacco, the PAL and Prx transgenes do not act additively in disease resistance. Stacking with Prx did not prevent a visible growth inhibition from PAL overexpression. Practical use of ShPAL will likely require more sophisticated developmental control, and we conclude that ShPx2 is a preferred candidate for development as a resistance transgene.
Keyword Plant defence
Genetic engineering
Genetic modification
Disease resistance
Lignin
Transgene stacking
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article
Collections: Official 2012 Collection
School of Agriculture and Food Sciences
 
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