Cytochrome P450 isozyme protein verified in the skin of southern hemisphere humpback whales (Megaptera novaeangliae): Implications for biochemical biomarker assessment

Waugh, Courtney A., Huston, Wilhelmina M., Noad, Michael J. and Bengtson Nash, Susan (2011) Cytochrome P450 isozyme protein verified in the skin of southern hemisphere humpback whales (Megaptera novaeangliae): Implications for biochemical biomarker assessment. Marine Pollution Bulletin, 62 4: 758-761.


Author Waugh, Courtney A.
Huston, Wilhelmina M.
Noad, Michael J.
Bengtson Nash, Susan
Title Cytochrome P450 isozyme protein verified in the skin of southern hemisphere humpback whales (Megaptera novaeangliae): Implications for biochemical biomarker assessment
Formatted title Cytochrome P450 isozyme protein verified in the skin of southern hemisphere humpback whales (Megaptera novaeangliae): Implications for biochemical biomarker assessment
Journal name Marine Pollution Bulletin   Check publisher's open access policy
ISSN 0025-326X
1879-3363
Publication date 2011-04
Sub-type Article (original research)
DOI 10.1016/j.marpolbul.2011.01.007
Volume 62
Issue 4
Start page 758
End page 761
Total pages 4
Place of publication Oxford, U.K.
Publisher Elsevier
Collection year 2012
Language eng
Formatted abstract Large mysticete whales represent a unique challenge for chemical risk assessment. Few epidemiological investigations are possible due to the low incidence of adult stranding events. Similarly their often extreme life-history adaptations of prolonged migration and fasting challenge exposure assumptions. Molecular biomarkers offer the potential to complement information yielded through tissue chemical analysis, as well as providing evidence of a molecular response to chemical exposure. In this study we confirm the presence of cytochrome P450 reductase (CPR) and cytochrome P450 isoenzyme 1A1 (CYP1A1) in epidermal tissue of southern hemisphere humpback whales (Megaptera novaeangliae). The detection of CYP1A1 in the integument of the humpback whale affords the opportunity for further quantitative non-destructive investigations of enzyme activity as a function of chemical stress.
Keyword Humpback whale
CYP1A1
Biomarkers
Western blot
Phoca-vitulina
CYP1A1
Expression
Induction
Exposure
Biopsy
P4501A1
Cetaceans
Receptor
Beluga
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 2 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 2 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Access Statistics: 23 Abstract Views  -  Detailed Statistics
Created: Mon, 20 Feb 2012, 16:21:58 EST by Dr Michael Noad on behalf of National Res Centre For Environmental Toxicology