Polarisation, key to good localisation

van Beest, Moniek, Robben, Joris H., Savelkoul, Paul J. M., Hendriks, Giel, Devonald, Mark A. J., Konings, Irene B. M., Lagendijk, Anne K., Karet, Fiona and Deen, Peter M. T. (2006) Polarisation, key to good localisation. BBA: Biomembranes, 1758 8: 1126-1133. doi:10.1016/j.bbamem.2006.03.007


Author van Beest, Moniek
Robben, Joris H.
Savelkoul, Paul J. M.
Hendriks, Giel
Devonald, Mark A. J.
Konings, Irene B. M.
Lagendijk, Anne K.
Karet, Fiona
Deen, Peter M. T.
Title Polarisation, key to good localisation
Journal name BBA: Biomembranes   Check publisher's open access policy
ISSN 0005-2736
Publication date 2006-08
Sub-type Article (original research)
DOI 10.1016/j.bbamem.2006.03.007
Volume 1758
Issue 8
Start page 1126
End page 1133
Total pages 8
Editor F. Chaumont
P. M. T. Deen
C. Delporte
O. Devuyst
J. -M. Verbavatz
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Abstract Polarisation of cells is crucial for vectorial transport of ions and solutes. In literature, however, proteins specifically targeted to the apical or basolateral membrane are often studied in non-polarised cells. To investigate whether these data can be extrapolated to expression in polarised cells, we studied several membrane-specific proteins. In polarised MDCK cells, the Aquaporin-2 water channel resides in intracellular vesicles and apical membrane, while the vasopressin-type 2 receptor, anion-exchanger 1 (AE1) protein and E-Cadherin mainly localise to the basolateral membrane. In non-polarised MDCK cells, however, Aquaporin-2 localises, besides plasma membrane, mainly in the Golgi complex, while the others show a dispersed staining throughout the cell. Moreover, while AQP2 mutants in dominant nephrogenic diabetes insipidus are missorted to different organelles in polarised cells, they all predominantly localise to the Golgi complex in non-polarised MDCK cells. Additionally, the maturation of V2R, and likely its missorting, is affected in transiently-transfected compared to stably-transfected cells. In conclusion, we show that the use of stably-transfected polarised cells is crucial in interpreting the processing and the localisation of membrane targeted proteins.
Keyword Polarisation
Aquaporin-2
V2R
Principal cell
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes Special Issue on Aquaporins

Document type: Journal Article
Sub-type: Article (original research)
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Created: Mon, 20 Feb 2012, 15:25:05 EST by Susan Allen on behalf of Institute for Molecular Bioscience