Targeted inhibition of miRNA maturation with morpholinos reveals a role for miR-375 in pancreatic islet development

Kloosterman, Wigard P., Lagendijk, Anne K., Ketting, Rene F., Moulton, Jon D. and Plasterk, Ronal H. A. (2007) Targeted inhibition of miRNA maturation with morpholinos reveals a role for miR-375 in pancreatic islet development. PLoS Biology, 5 8: 1738-1749. doi:10.1371/journal.pbio.0050203


Author Kloosterman, Wigard P.
Lagendijk, Anne K.
Ketting, Rene F.
Moulton, Jon D.
Plasterk, Ronal H. A.
Title Targeted inhibition of miRNA maturation with morpholinos reveals a role for miR-375 in pancreatic islet development
Journal name PLoS Biology   Check publisher's open access policy
ISSN 1544-9173
Publication date 2007-08-01
Sub-type Article (original research)
DOI 10.1371/journal.pbio.0050203
Open Access Status DOI
Volume 5
Issue 8
Start page 1738
End page 1749
Total pages 12
Place of publication San Francisco, CA, United States
Publisher Public Library of Science
Language eng
Formatted abstract
Several vertebrate microRNAs (miRNAs) have been implicated in cellular processes such as muscle differentiation, synapse function, and insulin secretion. In addition, analysis of Dicer null mutants has shown that miRNAs play a role in tissue morphogenesis. Nonetheless, only a few loss-of-function phenotypes for individual miRNAs have been described to date. Here, we introduce a quick and versatile method to interfere with miRNA function during zebrafish embryonic development. Morpholino oligonucleotides targeting the mature miRNA or the miRNA precursor specifically and temporally knock down miRNAs. Morpholinos can block processing of the primary miRNA (pri-miRNA) or the premiRNA, and they can inhibit the activity of the mature miRNA. We used this strategy to knock down 13 miRNAs conserved between zebrafish and mammals. For most miRNAs, this does not result in visible defects, but knockdown of miR-375 causes defects in the morphology of the pancreatic islet. Although the islet is still intact at 24 hours postfertilization, in later stages the islet cells become scattered. This phenotype can be recapitulated by independent control morpholinos targeting other sequences in the miR-375 precursor, excluding off-target effects as cause of the phenotype. The aberrant formation of the endocrine pancreas, caused by miR-375 knockdown, is one of the first lossof-function phenotypes for an individual miRNA in vertebrate development. The miRNA knockdown strategy presented here will be widely used to unravel miRNA function in zebrafish
Keyword Hormone-expressing cells
Zebrafish embryo
Enzyme dicer
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: ERA 2012 Admin Only
Institute for Molecular Bioscience - Publications
 
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Created: Tue, 21 Feb 2012, 01:19:34 EST by Susan Allen on behalf of Institute for Molecular Bioscience