The macromolecular state of A-kinase anchoring protein

Patel, Trushar R. and Winzor, Donald J. (2012) The macromolecular state of A-kinase anchoring protein. Journal of Molecular Recognition, 25 1: 11-14. doi:10.1002/jmr.1164

Author Patel, Trushar R.
Winzor, Donald J.
Title The macromolecular state of A-kinase anchoring protein
Journal name Journal of Molecular Recognition   Check publisher's open access policy
ISSN 0952-3499
Publication date 2012-01
Year available 2011
Sub-type Article (original research)
DOI 10.1002/jmr.1164
Volume 25
Issue 1
Start page 11
End page 14
Total pages 4
Editor Marc H. V. van Regenmortel
Place of publication Bognor Regis, West Sussex, U.K.
Publisher John Wiley & Sons
Collection year 2012
Language eng
Formatted abstract
The amendment of the interpretation of recently published size-exclusion chromatography (SEC) data for A-kinase anchoring protein (AKAP12) on Sephacryl-S400 has led to an increase in the estimated size of the supermolecular state from 840 to at least 6000 kDa. Although size-exclusion chromatography has sufficed to demonstrate unequivocally the existence of this 190-kDa scaffold protein in a supermolecular state, any quantitative estimate of the oligomer stoichiometry is shown to be precluded by failure of this empirical procedure to incorporate allowance for any deviation from globular shape—an important consideration in view of the extended structures exhibited by other extracellular matrix proteins.
Keyword A-kinase anchoring protein
Size-exclusion chromatography
Scaffold proteins
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
School of Chemistry and Molecular Biosciences
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Created: Mon, 20 Feb 2012, 08:56:07 EST by Lucy O'Brien on behalf of School of Chemistry & Molecular Biosciences