CD25(+) T-reg specifically suppress auto-Ab generation against pancreatic tissue autoantigens

Ludwig-Portugall, Isis, Hamilton-Williams, Emma E., Gotot, Janine and Kurts, Christian (2009) CD25(+) T-reg specifically suppress auto-Ab generation against pancreatic tissue autoantigens. European Journal of Immunology, 39 1: 225-233. doi:10.1002/eji.200838699


Author Ludwig-Portugall, Isis
Hamilton-Williams, Emma E.
Gotot, Janine
Kurts, Christian
Title CD25(+) T-reg specifically suppress auto-Ab generation against pancreatic tissue autoantigens
Formatted title
CD25+ Treg specifically suppress auto-Ab generation against pancreatic tissue autoantigens
Journal name European Journal of Immunology   Check publisher's open access policy
ISSN 0014-2980
1521-4141
Publication date 2009-01
Sub-type Article (original research)
DOI 10.1002/eji.200838699
Volume 39
Issue 1
Start page 225
End page 233
Total pages 9
Place of publication Weinheim, Germany
Publisher Wiley - V C H Verlag GmbH & Co. KGaA
Language eng
Formatted abstract
To study B-cell tolerance against non-lymphoid tissue autoantigens, we generated transgenic rat insulin promoter (RIP)-OVA/hen egg lysozyme (HEL) mice expressing the model antigens, OVA and HEL, in pancreatic islets. Their vaccination with OVA or HEL induced far less auto-Ab titers compared with non-transgenic controls. Depletion of CD25+ cells during immunization completely restored auto-Ab production, but did not affect antibodies against a foreign control antigen. Depletion at later time-points was not effective. OVA-specific CD25+ FoxP3+ Treg were more frequent in the autoantigen-draining pancreatic LN than in other secondary lymphatics of RIP-OVA/HEL mice. Consistently, B cells were suppressed in that LN and also in the spleen, which is known to concentrate circulating antigen, such as the antigens used for vaccination. Suppression involved preventing expansion of autoreactive B cells in response to autoantigen, reducing antibody production per B-cell and isotype changes. These findings demonstrate that CD25+ Treg suppress auto-Ab production against non-lymphoid tissue antigens in an antigen-specific manner.
Keyword Auto-antibodies
B cells
Tolerance
Transgenic mice
T-reg
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: ERA 2012 Admin Only
UQ Diamantina Institute Publications
 
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