Comparison of whole blood, serum, and plasma for early detection of candidemia by multiplex-tandem PCR

Lau, Anna, Halliday, Catriona, Chen, Sharon C.-A., Playford, E. Geoffrey, Stanley, Keith and Sorrell, Tania C. (2010) Comparison of whole blood, serum, and plasma for early detection of candidemia by multiplex-tandem PCR. Journal of Clinical Microbiology, 48 3: 811-816. doi:10.1128/JCM.01650-09

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Author Lau, Anna
Halliday, Catriona
Chen, Sharon C.-A.
Playford, E. Geoffrey
Stanley, Keith
Sorrell, Tania C.
Title Comparison of whole blood, serum, and plasma for early detection of candidemia by multiplex-tandem PCR
Journal name Journal of Clinical Microbiology   Check publisher's open access policy
ISSN 0095-1137
1098-660X
Publication date 2010-03
Sub-type Article (original research)
DOI 10.1128/JCM.01650-09
Open Access Status File (Publisher version)
Volume 48
Issue 3
Start page 811
End page 816
Total pages 6
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Language eng
Abstract We applied multiplex-tandem PCR (MT-PCR) to 255 EDTA whole-blood specimens, 29 serum specimens, and 24 plasma specimens from 109 patients with Candida bloodstream infection (candidemia) to determine whether a diagnosis could be expedited in comparison with the time to diagnosis by the use of standard blood culture. Overall, the MT-PCR performed better than blood culture with DNA extracted from whole blood from 52/74 (70%) patients, accelerating the time to detection (blood culture flagging) and determination of the pathogenic species (by use of the API 32C system [bioMérieux, Marcy l'Etoile, France]) by up to 4 days (mean, 2.2 days; range, 0.5 to 8 days). Candida DNA was detected more often in serum (71%) and plasma (75%) than in whole blood (54%), although relatively small numbers of serum and plasma specimens were tested. The sensitivity, specificity, positive predictive value, and negative predictive value of the assay with whole blood were 75%, 97%, 95%, and 85%, respectively. Fungal DNA was not detected by MT-PCR in 6/24 (25%) wholeblood samples drawn simultaneously with the positive blood culture sample. MT-PCR performed better with whole-blood specimens stored at -20° C (75%) and when DNA was extracted within 1 week of sampling (66%). The molecular and culture identification results correlated for 61 of 66 patients (92%); one discrepant result was due to misidentification by culture. All but one sample from 53 patients who were at high risk of candidemia but did not have proven disease were negative by MT-PCR. The results demonstrate the good potential of MT-PCR to detect candidemia, to provide Candida species identification prior to blood culture positivity, and to provide improved sensitivity when applied to with serum and plasma specimens.
Keyword Real-Time Pcr
Polymerase-Chain-Reaction
Germ Tube Test
Culture Bottles
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
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