Multiplex assay for species identification and monitoring of insecticide resistance in Anopheles punctulatus group populations of Papua New Guinea

Henry-Halldin, Cara N., Nadesakumaran, Kogulan, Keven, John Bosco, Zimmerman, Allison M., Siba, Peter, Mueller, Ivo, Hetzel, Manuel W., Kazura, James W., Thomsen, Edward, Reimer, Lisa J. and Zimmerman, Peter A. (2012) Multiplex assay for species identification and monitoring of insecticide resistance in Anopheles punctulatus group populations of Papua New Guinea. American Journal of Tropical Medicine and Hygiene, 86 1: 140-151. doi:10.4269/ajtmh.2012.11-0503


Author Henry-Halldin, Cara N.
Nadesakumaran, Kogulan
Keven, John Bosco
Zimmerman, Allison M.
Siba, Peter
Mueller, Ivo
Hetzel, Manuel W.
Kazura, James W.
Thomsen, Edward
Reimer, Lisa J.
Zimmerman, Peter A.
Title Multiplex assay for species identification and monitoring of insecticide resistance in Anopheles punctulatus group populations of Papua New Guinea
Formatted title
Multiplex assay for species identification and monitoring of insecticide resistance in Anopheles punctulatus group populations of Papua New Guinea
Journal name American Journal of Tropical Medicine and Hygiene   Check publisher's open access policy
ISSN 0002-9637
1476-1645
Publication date 2012-01
Sub-type Article (original research)
DOI 10.4269/ajtmh.2012.11-0503
Volume 86
Issue 1
Start page 140
End page 151
Total pages 12
Place of publication Deerfield, IL, United States
Publisher American Society of Tropical Medicine and Hygiene
Collection year 2013
Language eng
Formatted abstract
Anopheles punctulatus sibling species (An. punctulatus s.s., Anopheles koliensis, and Anopheles farauti species complex [eight cryptic species]) are principal vectors of malaria and filariasis in the Southwest Pacific. Given significant effort to reduce malaria and filariasis transmission through insecticide-treated net distribution in the region, effective strategies to monitor evolution of insecticide resistance among An. punctulatus sibling species is essential. Mutations in the voltage-gated sodium channel (VGSC) gene have been associated with knock-down resistance (kdr) to pyrethroids and DDT in malarious regions. By examining VGSC sequence polymorphism we developed a multiplex assay to differentiate wild-type versus kdr alleles and query intron-based polymorphisms that enable simultaneous species identification. A survey including mosquitoes from seven Papua New Guinea Provinces detected no kdr alleles in any An. punctulatus species. Absence of VGSC sequence introgression between species and evidence of geographic separation within species suggests that kdr must be monitored in each An. punctulatus species independently.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
School of Public Health Publications
 
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