Endometrial gene expression during early pregnancy differs between fertile and subfertile dairy cow strains

Walker, Caroline G., Littlejohn, Mathew D., Mitchell, Murray D., Roche, John R. and Meier, Susanne (2012) Endometrial gene expression during early pregnancy differs between fertile and subfertile dairy cow strains. Physiological Genomics, 44 1: 47-58. doi:10.1152/physiolgenomics.00254.2010


Author Walker, Caroline G.
Littlejohn, Mathew D.
Mitchell, Murray D.
Roche, John R.
Meier, Susanne
Title Endometrial gene expression during early pregnancy differs between fertile and subfertile dairy cow strains
Journal name Physiological Genomics   Check publisher's open access policy
ISSN 1094-8341
1531-2267
Publication date 2012-01
Year available 2011
Sub-type Article (original research)
DOI 10.1152/physiolgenomics.00254.2010
Volume 44
Issue 1
Start page 47
End page 58
Total pages 12
Place of publication Bethesda, MD, United States
Publisher American Physiological Society
Collection year 2012
Language eng
Formatted abstract
A receptive uterine environment is a key component in determining a successful reproductive outcome. We tested the hypothesis that endometrial gene expression patterns differ in fertile and subfertile dairy cow strains. Twelve lactating dairy cattle of strains characterized as having fertile (n = 6) and subfertile (n = 6) phenotypes underwent embryo transfer on day 7 of the reproductive cycle. Caruncular and intercaruncular endometrial tissue was obtained at day 17 of pregnancy, and microarrays used to characterize transcriptional profiles. Statistical analysis of microarray data at day 17 of pregnancy revealed 482 and 1,021 differentially expressed transcripts (P value < 0.05) between fertile and subfertile dairy cow strains in intercaruncular and caruncular tissue, respectively. Functional analysis revealed enrichment for several pathways involved in key reproductive processes, including the immune response to pregnancy, luteolysis, and support of embryo growth and development, and in particular, regulation of histotroph composition. Genes implicated in the process of immune tolerance to the embryo were downregulated in subfertile cows, as were genes involved in preventing luteolysis and genes that promote embryo growth and development. This study provides strong evidence that the endometrial gene expression profile may contribute to the inferior reproductive performance of the subfertile dairy cow strain.
Keyword Pregnancy recognition and maintenance
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online before print November 2011

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
Official 2012 Collection
 
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