Drosophila ribosomal protein mutants control tissue growth non-autonomously via effects on the prothoracic gland and ecdysone

Lin, Jane I., Mitchell, Naomi C., Kalcina, Marina, Tchoubrieva, Elly, Stewart, Mary J., Marygold, Steven J., Walker, Cherryl d., Thomas, George, Leevers, Sally J., Pearson, Richard B., Quinn, Leonie M. and Hannan, Ross D. (2011) Drosophila ribosomal protein mutants control tissue growth non-autonomously via effects on the prothoracic gland and ecdysone. Plos Genetics, 7 12: e1002408.1-e1002408.8. doi:10.1371/journal.pgen.1002408


Author Lin, Jane I.
Mitchell, Naomi C.
Kalcina, Marina
Tchoubrieva, Elly
Stewart, Mary J.
Marygold, Steven J.
Walker, Cherryl d.
Thomas, George
Leevers, Sally J.
Pearson, Richard B.
Quinn, Leonie M.
Hannan, Ross D.
Title Drosophila ribosomal protein mutants control tissue growth non-autonomously via effects on the prothoracic gland and ecdysone
Formatted title
Drosophila ribosomal protein mutants control tissue growth non-autonomously via effects on the prothoracic gland and ecdysone
Journal name Plos Genetics   Check publisher's open access policy
ISSN 1553-7390
1553-7404
Publication date 2011-12-01
Sub-type Article (original research)
DOI 10.1371/journal.pgen.1002408
Open Access Status DOI
Volume 7
Issue 12
Start page e1002408.1
End page e1002408.8
Total pages 18
Place of publication San Francisco, CA, United States
Publisher Public Library of Science
Collection year 2012
Language eng
Formatted abstract
The ribosome is critical for all aspects of cell growth due to its essential role in protein synthesis. Paradoxically, many Ribosomal proteins (Rps) act as tumour suppressors in Drosophila and vertebrates. To examine how reductions in Rps could lead to tissue overgrowth, we took advantage of the observation that an RpS6 mutant dominantly suppresses the small rough eye phenotype in a cyclin E hypomorphic mutant (cycE JP). We demonstrated that the suppression of cycE JP by the RpS6 mutant is not a consequence of restoring CycE protein levels or activity in the eye imaginal tissue. Rather, the use of UAS-RpS6 RNAi transgenics revealed that the suppression of cycE JP is exerted via a mechanism extrinsic to the eye, whereby reduced Rp levels in the prothoracic gland decreases the activity of ecdysone, the steroid hormone, delaying developmental timing and hence allowing time for tissue and organ overgrowth. These data provide for the first time a rationale to explain the counter-intuitive organ overgrowth phenotypes observed for certain members of the Minute class of Drosophila Rp mutants. They also demonstrate how Rp mutants can affect growth and development cell non-autonomously.
Keyword Diamond-blackfan anemia
Eye imaginal disc
Cyclin-E
Cell-proliferation
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Article number e1002408

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
School of Biomedical Sciences Publications
 
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