Moult cycle specific differential gene expression profiling of the crab Portunus pelagicus

Kuballa, Anna V., Holton, Timothy A., Paterson, Brian and Elizur, Abigail (2011) Moult cycle specific differential gene expression profiling of the crab Portunus pelagicus. BMC Genomics, 12 Article number 147: . doi:10.1186/1471-2164-12-147

Author Kuballa, Anna V.
Holton, Timothy A.
Paterson, Brian
Elizur, Abigail
Title Moult cycle specific differential gene expression profiling of the crab Portunus pelagicus
Journal name BMC Genomics   Check publisher's open access policy
ISSN 1471-2164
Publication date 2011-03
Sub-type Article (original research)
DOI 10.1186/1471-2164-12-147
Open Access Status DOI
Volume 12
Issue Article number 147
Total pages 19
Place of publication London, England, U.K.
Publisher BioMed Central
Collection year 2012
Language eng
Formatted abstract
Crustacean moulting is a complex process involving many regulatory pathways. A holistic approach to examine differential gene expression profiles of transcripts relevant to the moulting process, across all moult cycle stages, was used in this study. Custom cDNA microarrays were constructed for Portunus pelagicus. The printed arrays contained 5000 transcripts derived from both the whole organism, and from individual organs such as the brain, eyestalk, mandibular organ and Y-organ from all moult cycle stages.
A total of 556 clones were sequenced from the cDNA libraries used to construct the arrays. These cDNAs represented 175 singletons and 62 contigs, resulting in 237 unique putative genes. The gene sequences were classified into the following biological functions: cuticular proteins associated with arthropod exoskeletons, farnesoic acid O-methyltransferase (FaMeT), proteins belonging to the hemocyanin gene family, lectins, proteins relevant to lipid metabolism, mitochondrial proteins, muscle related proteins, phenoloxidase activators and ribosomal proteins. Moult cycle-related differential expression patterns were observed for many transcripts. Of particular interest were those relating to the formation and hardening of the exoskeleton, and genes associated with cell respiration and energy metabolism.
The expression data presented here provide a chronological depiction of the molecular events associated with the biological changes that occur during the crustacean moult cycle. Tracing the temporal expression patterns of a large variety of transcripts involved in the moult cycle of P. pelagicus can provide a greater understanding of gene function, interaction, and regulation of both known and new genes with respect to the moulting process.
Keyword Crustacean hyperglycemic hormone
Crayfish Procambarus-Clarkii
Farnesoic Acid Stimulation
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
Queensland Alliance for Agriculture and Food Innovation
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Citation counts: TR Web of Science Citation Count  Cited 23 times in Thomson Reuters Web of Science Article | Citations
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Created: Thu, 09 Feb 2012, 09:18:20 EST by Dr Timothy Holton on behalf of Qld Alliance for Agriculture and Food Innovation