Generation and characterization of LIF-dependent canine induced pluripotent stem cells from adult dermal fibroblasts

Whitworth, Deanne J., Ovchinnikov, Dmitry A. and Wolvetang, Ernst J. (2012) Generation and characterization of LIF-dependent canine induced pluripotent stem cells from adult dermal fibroblasts. Stem Cells and Development, 21 12: 2288-2297. doi:10.1089/scd.2011.0608


Author Whitworth, Deanne J.
Ovchinnikov, Dmitry A.
Wolvetang, Ernst J.
Title Generation and characterization of LIF-dependent canine induced pluripotent stem cells from adult dermal fibroblasts
Journal name Stem Cells and Development   Check publisher's open access policy
ISSN 1547-3287
1557-8534
Publication date 2012-08-10
Sub-type Article (original research)
DOI 10.1089/scd.2011.0608
Volume 21
Issue 12
Start page 2288
End page 2297
Total pages 10
Place of publication New Rochelle, NY, United States
Publisher Mary Ann Liebert
Collection year 2013
Language eng
Formatted abstract
Dogs provide a more clinically relevant model of human disease than rodents, particularly with respect to hereditary diseases. Thus, the availability of canine stem cells will greatly facilitate the use of the dog in the development of stem cell-based gene therapies and regenerative medicine. In this study we describe the production of canine induced pluripotent stem cells (ciPSCs) from adult dermal fibroblasts. These cells have a morphology resembling previously described canine embryonic stem cells, a normal karyotype, and express pluripotency markers including alkaline phosphatase, Nanog, Oct4, Telomerase, SSEA1, SSEA4, TRA1-60, TRA1-81, and Rex1. Furthermore, the inactive X chromosome is reactivated indicating a ground-state pluripotency. In culture they readily form embryoid bodies, which in turn give rise to cell types from all 3 embryonic germ layers, as indicated by expression of the definitive endoderm markers Cxcr4 and α-fetoprotein, mesoderm markers Collagen IIA and Gata2, and ectoderm markers βIII-tubulin, Enolase, and Nestin. Of particular significance is the observation that these ciPSCs are dependent only on leukemia inhibitory factor (LIF), making them similar to mouse and canine embryonic stem cells, but strikingly unlike the ciPSCs recently described in two other studies, which were dependent on both basic fibroblast growth factor and LIF in order to maintain their pluripotency. Thus, our ciPSCs closely resemble mouse ESCs derived from the inner cell mass of preimplantation embryos, while the previously described ciPSCs appear to be more representative of cells from the epiblast of mouse postimplantation embryos.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Online Ahead of Print: February 15, 2012

 
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Created: Wed, 08 Feb 2012, 18:10:40 EST by Dr Deanne Whitworth on behalf of School of Veterinary Science