Gene expression in the medulla following oral infection of cattle with bovine spongiform encephalopathy

Almeida, Luciane M., Basu, Urmila, Khaniya, Bina, Taniguchi, Masaaki, Williams, John L., Moore, Stephen S. and Guan, Le Luo (2011) Gene expression in the medulla following oral infection of cattle with bovine spongiform encephalopathy. Journal of Toxicology and Environmental Health Part A: Current Issues, 74 2-4: 110-126. doi:10.1080/15287394.2011.529061


Author Almeida, Luciane M.
Basu, Urmila
Khaniya, Bina
Taniguchi, Masaaki
Williams, John L.
Moore, Stephen S.
Guan, Le Luo
Title Gene expression in the medulla following oral infection of cattle with bovine spongiform encephalopathy
Journal name Journal of Toxicology and Environmental Health Part A: Current Issues   Check publisher's open access policy
ISSN 1528-7394
1087-2620
Publication date 2011
Sub-type Article (original research)
DOI 10.1080/15287394.2011.529061
Volume 74
Issue 2-4
Start page 110
End page 126
Total pages 7
Editor Neil R. Cashman
Shalu Darshan
Daniel Krewski
Michael G. Tyshenko
Place of publication Philadelphia, PA, United States
Publisher Taylor & Francis
Collection year 2012
Language eng
Formatted abstract The identification of variations in gene expression in response to bovine spongiform encephalopathy (BSE) may help to elucidate the mechanisms of neuropathology and prion replication and discover biomarkers for disease. In this study, genes that are differentially expressed in the caudal medulla tissues of animals infected with different doses of PrPBSE at 12 and 45 mo post infection were compared using array containing 24,000 oligonucleotide probes. Data analysis identified 966 differentially expressed (DE) genes between control and infected animals. Genes identified in at least two of four experiments (control versus 1-g infected animals at 12 and 45-mo; control versus 100-g infected animals at 12 and 45 mo) were considered to be the genes that may be associated with BSE disease. From the 176 DE genes associated with BSE, 84 had functions described in the Gene Ontology (GO) database. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of 14 genes revealed that prion infection may cause dysfunction of several different networks, including extracellular matrix (ECM), cell adhesion, neuroactive ligand–receptor interaction, complement and coagulation cascades, MAPK signaling, neurodegenerative disorder, SNARE interactions in vesicular transport, and the transforming growth factor (TGF) beta signaling pathways. The identification of DE genes will contribute to a better understanding of the molecular mechanisms of neuropathology in bovine species. Additional studies on larger number of animals are in progress in our laboratory to investigate the roles of these DE genes in pathogenesis of BSE.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes Special Issue: Prion Research in Perspective 2010

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
Queensland Alliance for Agriculture and Food Innovation
 
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Created: Wed, 08 Feb 2012, 12:49:19 EST by Stephen Moore on behalf of Qld Alliance for Agriculture and Food Innovation