The predominant role of IP3 type 1 receptors in activation of store-operated Ca2+ entry in liver cells

Jones, L., Ma, L., Castro, J., Litjens, T., Barritt, G. J. and Rychkov, G. Y. (2011) The predominant role of IP3 type 1 receptors in activation of store-operated Ca2+ entry in liver cells. BBA: Biomembranes, 1808 3: 745-751. doi:10.1016/j.bbamem.2010.12.013


Author Jones, L.
Ma, L.
Castro, J.
Litjens, T.
Barritt, G. J.
Rychkov, G. Y.
Title The predominant role of IP3 type 1 receptors in activation of store-operated Ca2+ entry in liver cells
Formatted title
The predominant role of IP3 type 1 receptors in activation of store-operated Ca2+ entry in liver cells
Journal name BBA: Biomembranes   Check publisher's open access policy
ISSN 0005-2736
0304-4157
Publication date 2011-03
Year available 2010
Sub-type Article (original research)
DOI 10.1016/j.bbamem.2010.12.013
Volume 1808
Issue 3
Start page 745
End page 751
Total pages 7
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Collection year 2012
Language eng
Formatted abstract
Physiologically, hormone induced release of Ca2+ from intracellular stores occurs in response to inositol 1,4,5-trisphosphate (IP3) binding to its receptors expressed on the membranes of intracellular organelles, mainly endoplasmic reticulum. These IP3 receptors act as channels, releasing Ca2+ into the cytoplasmic space where it is responsible for regulating a host of distinct cellular processes. The depletion of intracellular Ca2+ stores leads to activation of store-operated Ca2+ channels on the plasma membrane which replenishes lost Ca2+ and sustain Ca2+ signalling. There are three isoforms of IP3 receptor, each exhibiting distinctive properties, however, little is known about the role of each isoform in the activation of store-operated Ca2+ entry. Recent evidence suggest that at least in some cell types the endoplasmic reticulum is not a homogeneous Ca2+ store, and there might be a sub-compartment specifically linked to the activation of store-operated Ca2+ channels, and Ca2+ release activated Ca2+ (CRAC) channel in particular. Furthermore, this sub-compartment might express only certain types of IP3 receptor but not the others. Here we show that H4IIE liver cells express all three types of IP3 receptor, but only type 1 and to a lesser extent type 3, but not type 2, participate in the activation of CRAC current (ICRAC), while type 1 and type 2, but not type 3, participate in observed Ca2+ release in response to receptor stimulation. Presented results suggest that in H4IIE rat liver cells the sub-compartment of intracellular Ca2+ store linked to the activation of ICRAC predominantly expresses type 1 IP3 receptors.

Research Highlights: ►H4IIE liver cells express all three types of IP3 receptor. ►IP3R type 1 and to a lesser extent type 3, but not type 2, participate in activation of ICRAC. ►IP3R type 1 and type 2, but not type 3, participate in observed Ca2+ release in response to receptor stimulation. ►In H4IIE rat liver cells the sub-compartment of intracellular Ca2+ store linked to activation of ICRAC predominantly expresses type 1 IP3 receptors.
Keyword CRAC channels
IP3 receptor
Ca2+ stores
Inositol 1,4,5-trisphosphate receptor
Capacitative calcium-entry
Endoplasmic-reticulum
Trisphosphate receptors,
InsP(3) receptors
Rat hepatocytes
Channels
Release
Membrane
Isoforms
InsP3 receptors
Ca(2+) stores
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status Non-UQ
Additional Notes Available online 20 December 2010.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
Institute for Molecular Bioscience - Publications
 
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Created: Tue, 07 Feb 2012, 11:56:31 EST by Susan Allen on behalf of Institute for Molecular Bioscience