Identification of constraining experimental-design factors in mycorrhizal pot-growth studies

Audet, Patrick and Charest, Christiane (2010) Identification of constraining experimental-design factors in mycorrhizal pot-growth studies. Journal of Botany, 2010 718013.1-718013.6. doi:10.1155/2010/718013

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Author Audet, Patrick
Charest, Christiane
Title Identification of constraining experimental-design factors in mycorrhizal pot-growth studies
Journal name Journal of Botany   Check publisher's open access policy
ISSN 2090-0120
2090-0139
Publication date 2010
Sub-type Article (original research)
DOI 10.1155/2010/718013
Open Access Status DOI
Volume 2010
Start page 718013.1
End page 718013.6
Total pages 6
Place of publication New York, NY, United States
Publisher Hindawi Publishing Corporation
Language eng
Abstract In the objective of testing the design of pot-growth experiments, we conducted two greenhouse studies of a “dwarf ” sunflower cultivar and an arbuscular mycorrhizal (AM) fungus to determine how pot size and inoculum distribution affect plant growth and AM symbiosis. As predicted, large-potted plants developed a greater overall biomass and root colonization than smallpotted ones which we attributed to the larger “rootable” volume. Furthermore, plants grown in a band of high density inoculum substrate showed a higher prevalence of fungal vesicles (sites of lipid storage) indicating amore advanced level of root colonization compared to those grown in a dispersed inoculum substrate; this likely being due to the higher frequency of interaction between roots and fungal propagules. In a second experiment, large-potted AM plants showed a greater tolerance to water deficit than non-AM control plants; however, this mycorrhizal effect was not detected among small-potted plants. We conclude that careful consideration should be made toward design parameters to limit result biases and ultimately facilitate comparison of findings between studies.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes Attached pdf - Copyright © 2010 Patrick Audet and Christiane Charest. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Centre for Mined Land Rehabilitation Publications
ERA 2012 Admin Only
 
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Created: Tue, 07 Feb 2012, 11:30:02 EST by Dr Patrick Audet on behalf of Centre For Mined Land Rehabilitation