Magnetic silica spheres with large nanopores for nucleic acid adsorption and cellular uptake

Liu, Jian, Wang, Bo, Hartono, Sandy Budi, Liu, Tingting, Kantharidis, Phillip, Middelberg, Anton P. J., Lu, Gao Qing (Max), He, Lizhong and Qiao, Shi Zhang (2012) Magnetic silica spheres with large nanopores for nucleic acid adsorption and cellular uptake. Biomaterials, 33 3: 970-978. doi:10.1016/j.biomaterials.2011.10.001

Author Liu, Jian
Wang, Bo
Hartono, Sandy Budi
Liu, Tingting
Kantharidis, Phillip
Middelberg, Anton P. J.
Lu, Gao Qing (Max)
He, Lizhong
Qiao, Shi Zhang
Title Magnetic silica spheres with large nanopores for nucleic acid adsorption and cellular uptake
Journal name Biomaterials   Check publisher's open access policy
ISSN 0142-9612
Publication date 2012-01
Year available 2011
Sub-type Article (original research)
DOI 10.1016/j.biomaterials.2011.10.001
Volume 33
Issue 3
Start page 970
End page 978
Total pages 9
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Collection year 2012
Language eng
Formatted abstract
Template assisted fabrication of magnetic silica nanospheres with large nanopores (MSNLP) and their adsorption and delivery of nucleic acids are reported in this paper. Silica spheres with controlled particle diameter (∼400 nm) and large nanopore size (13–24 nm) are prepared by using Brij56 as a template of mesopore, enabling incorporation of magnetic nanocrystals into the particles under mild neutral synthesis conditions. High resolution transmission electron microscopy (HRTEM), X-ray diffraction (XRD), and field-dependent magnetisation measurements confirm that the magnetic nanocrystals have been encapsulated into the silica spheres. The saturation magnetisation values of the resulted magnetic-silica nanocomposites are tunable by adjusting the amount of Fe3O4 magnetic nanocrystals used in the synthesis process. The nitrogen sorption analysis reveals that mesopores with large pore size exist in the silica matrix. After functionalisation of the silica surface with poly-(l-lysine) (PLL), the nanoparticles show strong adsorption capacity (qm ranging from 10 to 22.5 μg/mg) for CpG DNA. We have further demonstrated successful delivery of miRNA into rat proximal tubular epithelial cells, facilitated by efficient cellular uptake of the nanocomposites. This work provides a convenient strategy to prepare MSNLP which can offer a versatile platform for biological applications such as simultaneous drug delivery and magnetic resonance imagining under external magnetic field.
Keyword Magnetic-silica nanocomposites
Mesoporous materials
Cellular uptake
CpG DNA adsorption
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online 22 October 2011

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