Epithelial cell coculture models for studying infectious diseases: Benefits and limitations

Duell, Benjamin, Cripps, Allan W., Schembri, Mark A. and Ulett, Glen C. (2011) Epithelial cell coculture models for studying infectious diseases: Benefits and limitations. Journal of Biomedicine and Biotechnology, 2011 1-9. doi:10.1155/2011/852419


Author Duell, Benjamin
Cripps, Allan W.
Schembri, Mark A.
Ulett, Glen C.
Title Epithelial cell coculture models for studying infectious diseases: Benefits and limitations
Journal name Journal of Biomedicine and Biotechnology   Check publisher's open access policy
ISSN 1110-7243
1110-7251
2314-6141
2314-6133
Publication date 2011
Sub-type Critical review of research, literature review, critical commentary
DOI 10.1155/2011/852419
Open Access Status DOI
Volume 2011
Start page 1
End page 9
Total pages 9
Place of publication New York, NY, United States
Publisher Hindawi Publishing Corporation
Collection year 2012
Language eng
Abstract Countless in vitro cell culture models based on the use of epithelial cell types of single lineages have been characterized and have provided insight into the mechanisms of infection for various microbial pathogens. Diverse culture models based on diseaserelevant mucosal epithelial cell types derived from gastrointestinal, genitourinary, and pulmonary organ systems have delineated many key host-pathogen interactions that underlie viral, parasitic, and bacterial disease pathogenesis. An alternative to single lineage epithelial cell monoculture, which offers more flexibility and can overcome some of the limitations of epithelial cell culture models based on only single cell types, is coculture of epithelial cells with other host cell types. Various coculture models have been described, which incorporate epithelial cell types in culture combination with a wide range of other cell types including neutrophils, eosinophils, monocytes, and lymphocytes. This paper will summarize current models of epithelial cell coculture and will discuss the benefits and limitations of epithelial cell coculture for studying host-pathogen dynamics in infectious diseases.
Keyword Group B Streptococcus
Uropathogenic Escherichia Coli
Host Pathogen Interactions
Uroplakin Gene Expression
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Critical review of research, literature review, critical commentary
Collections: Official 2012 Collection
School of Chemistry and Molecular Biosciences
 
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