Residues in domain III of the dengue virus envelope glycoprotein involved in cell-surface glycosaminoglycan binding

Watterson, Daniel, Kobe, Bostjan and Young, Paul R. (2012) Residues in domain III of the dengue virus envelope glycoprotein involved in cell-surface glycosaminoglycan binding. Journal of General Virology, 93 72-82. doi:10.1099/vir.0.037317-0


Author Watterson, Daniel
Kobe, Bostjan
Young, Paul R.
Title Residues in domain III of the dengue virus envelope glycoprotein involved in cell-surface glycosaminoglycan binding
Journal name Journal of General Virology   Check publisher's open access policy
ISSN 0022-1317
1465-2099
Publication date 2012-01
Sub-type Article (original research)
DOI 10.1099/vir.0.037317-0
Open Access Status
Volume 93
Start page 72
End page 82
Total pages 11
Place of publication Reading, United Kingdom
Publisher Society for General Microbiology
Collection year 2013
Language eng
Abstract The dengue virus (DENV) envelope (E) protein mediates virus entry into cells via interaction with a range of cell-surface receptor molecules. Cell-surface glycosaminoglycans (GAGs) have been shown to play an early role in this interaction, and charged oligosaccharides such as heparin bind to the E protein. We have examined this interaction using site-directed mutagenesis of a recombinant form of the putative receptor-binding domain III of the DENV-2E protein expressed as an MBP (maltose-binding protein)-fusion protein. Using an ELISA-based GAG-binding assay, cell-based binding analysis and antiviral-activity assays, we have identified two critical residues, K291 and K295, that are involved in GAG interactions. These studies have also demonstrated differential binding between mosquito and human cells.
Keyword Japanese Encephalitis Virus
West Nile Virus
Human Dendritic Cells
Membrane Fusion
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2013 Collection
School of Chemistry and Molecular Biosciences
Institute for Molecular Bioscience - Publications
 
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Created: Mon, 06 Feb 2012, 17:18:03 EST by System User on behalf of School of Chemistry & Molecular Biosciences