Formation of intracellular concentration landscapes by multisite protein modification

Munoz-Garcia, Javier, Kholodenko, Boris N. and Neufeld, Zoltan (2010) Formation of intracellular concentration landscapes by multisite protein modification. Biophysical Journal, 99 1: 59-66. doi:10.1016/j.bpj.2010.04.014

Author Munoz-Garcia, Javier
Kholodenko, Boris N.
Neufeld, Zoltan
Title Formation of intracellular concentration landscapes by multisite protein modification
Journal name Biophysical Journal   Check publisher's open access policy
ISSN 0006-3495
Publication date 2010-07
Sub-type Article (original research)
DOI 10.1016/j.bpj.2010.04.014
Volume 99
Issue 1
Start page 59
End page 66
Total pages 8
Place of publication St. Louis, MO, United States
Publisher Cell Press
Language eng
Abstract Multiple cellular proteins are covalently modified (e.g., phosphorylated/dephosphorylated) at several sites, which leads to diverse signaling activities. Here, we consider a signaling cascade that is activated at the plasma membrane and composed of two-site protein modification cycles, and we focus on the radial profile of the concentration landscapes created by different protein forms in the cytoplasm. We show that under proper conditions, the concentrations of modified proteins generate a series of peaks that propagate into the cell interior. Proteins modified at both sites form activity gradients with long plateaus that abruptly decay at successive locations along the path from the membrane to the nucleus. We demonstrate under what conditions signals generated at the membrane stall in the vicinity of that membrane or propagate into the cell. We derive analytical approximations for the main characteristics of the protein concentration profiles and demonstrate what we believe to be a novel steady-state pattern formation mechanism capable of generating precise spatial guidance for diverse cellular processes.
Keyword Signal-transduction
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Mathematics and Physics
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Created: Tue, 31 Jan 2012, 10:38:34 EST by Kay Mackie on behalf of School of Mathematics & Physics